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Anti-Tetrsapanin-3 (extracellular) Antibody

TSPAN3, Transmembrane 4 Superfamily Member 8, TM4SF8

Cat #: ANR-185
Alternative Name TSPAN3, Transmembrane 4 Superfamily Member 8, TM4SF8
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h, m, r
Immunogen
  • Peptide (C)DWENTDWFKETKNQS, corresponding to amino acid residues 155 - 169 of human Tetraspanin-3 (Accession O60637). Extracellular, 2nd loop.
Accession (Uniprot) Number O60637
Gene ID 10099
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Mouse, rat – identical
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.8 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Preadsorption Control 1 µg peptide per 1 µg antibody
Standard quality control of each lot Western blot analysis.
Applications: ifc, ih, lci, wb
May also work in: ic*, ip*
Western blot
  • Western blot analysis of rat brain lysates (lanes 1 and 3) and mouse brain lysates (lanes 2 and 4):
    Western blot analysis of rat brain lysates (lanes 1 and 3) and mouse brain lysates (lanes 2 and 4):
    1-2. Anti-Tetraspanin-3 (extracellular) Antibody (#ANR-185), (1:400).
    3-4. Anti- Tetraspanin-3 (extracellular) Antibody, preincubated with Tetraspanin-3 (extracellular) Blocking Peptide (BLP-NR185).
  • Western blot analysis of human K562 erythroleukemia cell line lysate (lanes 1 and 4), human Jurkat T-cell leukemia cell line lysate (lanes 2 and 5) and human LNCaP prostate adenocarcinoma cell line lysate (lanes 3 and 6):
    Western blot analysis of human K562 erythroleukemia cell line lysate (lanes 1 and 4), human Jurkat T-cell leukemia cell line lysate (lanes 2 and 5) and human LNCaP prostate adenocarcinoma cell line lysate (lanes 3 and 6):
    1-3. Anti-Tetraspanin-3 (extracellular) Antibody (#ANR-185), (1:200).
    4-6. Anti- Tetraspanin-3 (extracellular) Antibody, preincubated with Tetraspanin-3 (extracellular) Blocking Peptide (BLP-NR185).
Immunohistochemistry
  • Expression of Tetraspanin-3 in rat cortex.
    Expression of Tetraspanin-3 in rat cortex.
    Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-Tetraspanin-3 (extracellular) Antibody (#ANR-185), (1:300), followed by donkey anti-rabbit-biotin and streptavidin-Cy3. A. Tetraspanin-3 immunoreactivity (red) appears in neuron profiles (arrows). B. Pre-incubation of the antibody with Tetraspanin-3 (extracellular) Blocking Peptide (BLP-NR185), suppressed staining. Cell nuclei are stained with DAPI (blue).
Indirect flow cytometry
  • Cell surface detection of Tetraspanin-3 by indirect flow cytometry in live intact human THP-1 monocytic leukemia cell line:
    Cell surface detection of Tetraspanin-3 by indirect flow cytometry in live intact human THP-1 monocytic leukemia cell line:
    ___ Cells.
    ___ Cells + goat-anti-rabbit-FITC.
    ___ Cells + Anti-Tetraspanin-3 (extracellular) Antibody (#ANR-185), (2.5μg) + goat-anti-rabbit-FITC.
References
  1. Termini & Gillette (2017), Front Cell Dev Biol, 5, 34.
  2. Seipold et al (2017), Biochim Biophys Acta Mol Cell Res, 1864, 217.
  3. Tokoro, I. et al (2001), Biochem Biophys Res Commun, 288, 178.
Scientific background

Tetraspanin-3, encoded by TSPAN3 gene is a membrane-spanning glycoprotein that has a role in cell migration, proliferation, and interactions between cells and extracellular matrix.

As suggested by the family name, TSPANs are categorized by the presence of four trans-membrane domains, of which two are extracellular. The intracellular part includes the C and N terminal domains that can facilitate protein–protein interactions, as well as guide intracellular trafficking. TSPANs have multiple roles In the immune and vascular systems, and they act as organizers of functional microdomains and multi-subunit receptor complexes 1.

Tspan3 is highly expressed in the brain and elevated in brains of Alzheimer´s disease (AD) patients. It was shown to interact with ADAM10, which is a marker for Alzheimer's disease 2. It was also shown to be expressed by dendritic cells (DCs) which suggests that it may be involved in the function of DCs in association with T cell stimulation 3.

Its functional diversity and importance for the development and onset of various diseases makes Tetraspanin 3 an appealing research target.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 03/05/2021

Anti-Tetraspanin-3 (extracellular) Antibody (#ANR-185) is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot, immunohistochemistry and flow cytometry applications. It has been designed to recognize Tetraspanin-3 from rat, mouse and human samples.

For research purposes only, not for human use
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