Name: Anti-Thromboxane A2 Receptor Antibody
Brand: Alomone Labs
SKU: APR-069

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Overview

Cat #: APR-069

Alternative Name Prostanoid TP receptor, TXA2-R, TBXA2R

Lyophilized Powder yes

Type: Polyclonal

Host: Rabbit

Reactivity: h, m, r

Immunogen

Peptide (C)HAREATQRPRD(S)EVE, corresponding to amino acid residues 225-239 of rat TBXA2R (Accession P34978). 3^rd intracellular loop.

Accession (Uniprot) Number P34978

Gene ID 24816

Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.

Homology Rat – 14/15 amino acid residues identical; mouse – 13/15 amino acid residues identical; human – 12/15 amino acid residues identical.

RRID AB_2756764.

Purity Affinity purified on immobilized antigen.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN₃.

Isotype Rabbit IgG.

Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.

Antibody concentration after reconstitution 0.8 mg/ml.

Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Standard quality control of each lot Western blot analysis.

Applications: if, ih, wb

May also work in: ic*, ifc*, ip*

Western blot

Western blot analysis of mouse (lanes 1 and 3) and rat (lanes 2 and 4) brain lysates:
1,2. Anti-Thromboxane A2 Receptor Antibody (#APR-069), (1:200).
3,4. Anti-Thromboxane A2 Receptor Antibody, preincubated with Thromboxane A2 Receptor Blocking Peptide (#BLP-PR069).
Western blot analysis of human THP-1 monocytic leukemia (lanes 1 and 3) and human MEG-01 megakaryoblastic leukemia (lanes 2 and 4) cell line lysates:
1,2. Anti-Thromboxane A2 Receptor Antibody (#APR-069), (1:200).
3,4. Anti-Thromboxane A2 Receptor Antibody, preincubated with Thromboxane A2 Receptor Blocking Peptide (#BLP-PR069).

Immunohistochemistry

Expression of thromboxane A2 receptor in mouse cortex and hippocampus
Immunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-Thromboxane A2 Receptor Antibody (#APR-069), (1:200), followed by goat anti-rabbit-AlexaFluor-488. A. TBXA2R immunoreactivity (green) is observed in mouse parietal cortex neurons (arrows). B. TBXA2R immunoreactivity (green) is detected in neurons (horizontal arrows) of mouse hippocampal CA3 pyramidal layer (P). Cell nuclei are stained with DAPI (blue).

References

Yan, A. et al. (2017) Mol. Med. Rep. 16, 2491.
Zhao, Z. et al. (2017) Oncotarget 8, 30030.
Kinsella, B.T. (2016) Wilms Tumor, Brisbane (AU): Codon Publications, Chapter 11.

Scientific background

Thromboxane A2 (TxA2) is a prostanoid that is synthesized from arachidonic acid and plays an important role in haemostasis, a process regulating platelet aggregation and vascular tone. Thromboxane A2 binds to the thromboxane A2 receptor (TBXA2R) to induce strong vasoconstriction in renal arteries.

Thromboxane A2 receptor is a seven-transmembrane G-protein coupled receptor localized on the cell membrane and in intracellular compartments. TBXA2R is expressed in the central nervous system in several types of cells, such as microglia, oligodendrocytes and astrocytes. Imbalances in the levels of the receptor is widely implicated in several cardiovascular, pulmonary, renal and prostate pathologies^1-3.

Thromboxane A2 receptor mediates vasoconstriction and ischemia and its activation is implicated in atherosclerosis and inflammation. Activation of the receptor induces endothelial cell apoptosis by inhibiting Akt phosphorylation and also inhibits endothelial cell migration and angiogenesis by decreasing Akt and eNOS phosphorylation^1-3.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat

Lyophilized Powder

Anti-Thromboxane A2 Receptor Antibody (#APR-069) is a highly specific antibody directed against an epitope of the rat TBXA2R. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize TBXA2R from rat, mouse, and human samples.

Certificate of Analysis SDS

For research purposes only, not for human use

Last Update: 03/01/2024

Applications

Citations

Specifications

Scientific Background

Specific Control Product

Thromboxane A2 Receptor Blocking Peptide (#BLP-PR069) is the original antigen used for immunization during Anti-Thromboxane A2 Receptor Antibody (#APR-069) generation. The blocking peptide binds and ‘blocks’ Anti-Thromboxane A2 Receptor primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.

Thromboxane A2 Receptor Blocking Peptide (#BLP-PR069)

Shipping and Ordering Information

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Anti-Thromboxane A2 Receptor Antibody