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Anti-TMEM119 (extracellular) Antibody

Transmembrane Protein 119, Osteoblast induction factor, OBIF
Cat #: ANR-175
Alternative Name Transmembrane Protein 119, Osteoblast induction factor, OBIF
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: m, r
Immunogen
  • Peptide (C)GTPAFSPTPERPQPT, corresponding to amino acid residues 53 - 67 of mouse TMEM119 (Accession Q8R138). Extracellular, N-terminus.
Accession (Uniprot) Number Q8R138
Gene ID 231633
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Rat – 12/15 amino acid residues identical.
RRID AB_2876844
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG
Specificity This antibody will not recognize human TMEM119.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.8 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: ifc, wb
May also work in: ic*, ih*, ip*, lci*
Western blot
  • Western blot analysis of mouse brain membranes (lanes 1 and 3) and rat brain membranes (lanes 2 and 4):
    Western blot analysis of mouse brain membranes (lanes 1 and 3) and rat brain membranes (lanes 2 and 4):
    1, 2. Anti-TMEM119 (extracellular) Antibody (#ANR-175), (1:200).
    3, 4. Anti-TMEM119 (extracellular) Antibody, preincubated with TMEM119 (extracellular) Blocking Peptide (#BLP-NR175).
  • Western blot analysis mouse BV-2 microglia cell lysate:
    Western blot analysis mouse BV-2 microglia cell lysate:
    1. Anti-TMEM119 (extracellular) Antibody (#ANR-175), (1:200).
    2. Anti-TMEM119 (extracellular) Antibody, preincubated with TMEM119 (extracellular) Blocking Peptide (#BLP-NR175).
Indirect flow cytometry
  • Cell surface detection of TMEM119 in live intact mouse BV-2 microglia cell line:
    Cell surface detection of TMEM119 in live intact mouse BV-2 microglia cell line:
    ___ Cells.
    ___ Cells + goat-anti-rabbit-FITC.
    ___ Cells + Anti-TMEM119 (extracellular) Antibody (#ANR-175), (2.5µg) + goat-anti-rabbit-FITC.
References
  1. Bennett, M.L. et al. (2016) Proc. Natl. Acad. Sci. U.S.A. 113, E1738.
  2. Tanaka, K. et al. (2012) Bone 51, 158.
  3. van Wageningen, T.A. et al. (2019) Acta Neuropathol. Commun. 7, 206.
  4. Haage, V. et al. (2019) Acta Neuropathol. Commun. 7, 20.
Scientific background

Transmembrane protein 119, Tmem119, is a bone formation related factor that belongs to a group of bone morphogenetic proteins (BMPs). It is a class IA single pass transmembrane protein1.

Tmem119 promotes the differentiation of myoblasts (embryonic precursors of muscle cells) into osteoblasts and plays a role in numerous BMP signaling pathways1,2.

Tmem119 is uniquely expressed in microglia cells and it is therefore used as a microglia-specific homeostatic marker that can assist in differentiating microglia cells from peripherally derived macrophages in the brain3. This marker is very important for diagnosing CNS disease: monocytes/macrophages are key cellular modulators of brain homeostasis. In the healthy brain resident microglia are the predominant macrophage cell population but when the blood-brain barrier is leaking peripheral monocytes/macrophages can infiltrate the brain and participate in CNS disease pathogenesis. Distinguishing these two monocytesmacrophages populations is often challenging and hence the existence of a highly expressed and robust microglia marker such as Tmem119 is very important4.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat

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Last update: 20/10/2020

Anti-TMEM119 (extracellular) Antibody (#ANR-175) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot and indirect live cell flow cytometry. It has been designed to recognize TMEM119 from rat and mouse samples. The antibody will not recognize the protein from human samples.

For research purposes only, not for human use
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