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Anti-TMEM119 (extracellular)-FITC Antibody

Transmembrane Protein 119, Osteoblast induction factor, OBIF

Cat #: ANR-175-F
Alternative Name Transmembrane Protein 119, Osteoblast induction factor, OBIF
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: m, r
Immunogen
  • Peptide (C)GTPAFSPTPERPQPT, corresponding to amino acid residues 53 - 67 of mouse TMEM119 (Accession Q8R138). Extracellular, N-term.
Accession (Uniprot) Number Q8R138
Gene ID Tmem119
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Rat – 12 out of 15 amino acid residues identical. This antibody will not recognize human TMEM119
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG
Specificity This antibody will not recognize human TMEM119
Label Fluorescein isothiocyanate (FITC)
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 50 µl double distilled water (DDW).
Antibody concentration after reconstitution 1 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 × g 5 min).
Standard quality control of each lot Western blot analysis (unlabeled antibody, #ANR-175), and direct flow cytometry (labeled antibody).
Applications: fc
May also work in: cbe*, ic*
Direct flow cytometry
  • Cell surface detection of TMEM119 by direct flow cytometry in live intact mouse BV-2 microglia cells:
    Cell surface detection of TMEM119 by direct flow cytometry in live intact mouse BV-2 microglia cells:
    ___ Cells
    ___ Cells + Rabbit IgG isotype control-FITC
    ___ Cells + Anti-TMEM119 (extracellular)-FITC Antibody (ANR-175-F), 2.5µg.
References
  1. Bennett, M.L. et al. (2016) Proc. Natl. Acad. Sci. U.S.A. 113, E1738.
  2. Tanaka, K. et al. (2012) Bone 51, 158.
  3. van Wageningen, T.A. et al. (2019) Acta Neuropathol. Commun. 7, 206.
  4. Haage, V. et al. (2019) Acta Neuropathol. Commun. 7, 20.
Scientific background

Transmembrane protein 119, Tmem119, is a bone formation related factor that belongs to a group of bone morphogenetic proteins (BMPs). It is a class IA single pass transmembrane protein1.

Tmem119 promotes the differentiation of myoblasts (embryonic precursors of muscle cells) into osteoblasts and plays a role in numerous BMP signaling pathways1,2.

Tmem119 is uniquely expressed in microglia cells and it is therefore used as a microglia-specific homeostatic marker that can assist in differentiating microglia cells from peripherally derived macrophages in the brain3. This marker is very important for diagnosing CNS disease: monocytes/macrophages are key cellular modulators of brain homeostasis. In the healthy brain resident microglia are the predominant macrophage cell population but when the blood-brain barrier is leaking peripheral monocytes/macrophages can infiltrate the brain and participate in CNS disease pathogenesis. Distinguishing these two monocytesmacrophages populations is often challenging and hence the existence of a highly expressed and robust microglia marker such as Tmem119 is very important4.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 25/07/2021

Anti-TMEM119 (extracellular) Antibody (#ANR-175) is a highly specific antibody directed against an extracellular epitope of the mouse protein. The antibody can be used in western blot and indirect live cell flow cytometry. It has been designed to recognize TMEM119 from rat and mouse samples. The antibody will not recognize the protein from human samples.

Anti-TMEM119 (extracellular)-FITC Antibody (#ANR-175-F) is directly conjugated to fluorescein isothiocyanate (FITC) fluorophore. This conjugated antibody has been developed to be used in immunofluorescent applications such as direct flow cytometry and live cell imaging.

Polyclonal

For research purposes only, not for human use
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