Anti-TREM2 (extracellular)-FITC Antibody

Triggering receptor expressed on myeloid cells 2, Triggering receptor expressed on monocytes 2
  • New
Cat #: ANR-018-F
Alternative Name Triggering receptor expressed on myeloid cells 2, Triggering receptor expressed on monocytes 2
  • Lyophilized Powder
  • Type: Polyclonal
    Host: Rabbit
    Reactivity: h, m, r
      • Peptide (C)KHWGRRKAW(S)RQLGE, corresponding to amino acid residues 42-56 of mouse TREM2 (Accession Q99NH8). Extracellular, N-terminus.
        Anti-TREM2 (extracellular)-FITC Antibody
    Accession (Uniprot) Number Q99NH8
    Gene ID 83433
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Mouse, human – 14/15 amino acid residues identical; rat – 12/15 amino acid residues identical.
    Purity Affinity purified on immobilized antigen.
    Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Isotype Rabbit IgG.
    Label Fluorescein isothiocyanate (FITC).
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 15 µl or 50 µl double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 1 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 × g 5 min).
    Standard quality control of each lot Western blot analysis (unlabeled antibody, #ANR-018), and direct flow cytometry (labeled antibody).
    Applications: fc
    May also work in: ic*, lci*
      • Anti-TREM2 (extracellular)-FITC Antibody
        Cell surface detection of TREM2 in live intact mouse BV-2 microglia cells:
        ___ Cells.
        ___ Cells + rabbit IgG isotype control-FITC.
        ___ Cells + Anti-TREM2 (extracellular)-FITC Antibody (#ANR-018-F), (5 µg).
        Anti-TREM2 (extracellular)-FITC Antibody
        Cell surface detection of TREM2 in live intact human THP-1 monocytic leukemia cells:
        ___ Cells.
        ___ Cells + rabbit IgG isotype control-FITC.
        ___ Cells + Anti-TREM2 (extracellular)-FITC Antibody (#ANR-018-F), (2.5 µg).
    References
      • TREM2 (triggering receptor expressed on myeloid cells-2) is a type I transmembrane microglial surface receptor that is found to be expressed on myeloid cells including monocyte-derived dendritic cells, osteoclasts and bone-marrow derived macrophage. TREM2 protein transduces intracellular signals that regulate microglial functions such as cytokine production, migration, proliferation, phagocytosis, cell survival, synapse elimination, and compaction of amyloid plaques1,2.

        The TREM2 structure includes an immunoglobulin-like extracellular domain responsible for ligand binding, a transmembrane region and a short cytoplasmatic tail. The protein also includes an ectodomain that binds anionic and zwitterionic lipids, apolipoproteins and lipoprotein particles2,3.

        Studies show that TREM2 knockout mice display decreased levels of inflammatory cytokines. TREM2 has been shown to control two signaling pathways in the brain: regulation of phagocytosis and suppression of inflammatory reactivity. Nasu-Hakola disease, a rare and fatal disease, and polycystic lipomembranous osteodysplasia with sclerosing leukoencephalopathy has been found to be linked to homozygous loss-of-function mutations in TREM2 protein1,2. Studies also show that variants of TREM2 are associated with Alzheimer’s disease2,3.

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IFC- Indirect flow cytometry, IF- Immunofluorescence, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Last update: 17/10/2019

    Anti-TREM2 (extracellular) Antibody (#ANR-018) is a highly selective antibody directed against an epitope of the mouse protein. The antibody can be used in western blot and live cell flow cytometry applications. The antibody recognizes an extracellular epitope and can be used to detect the protein in living cells. It has been designed to recognize TREM2 from mouse, human, and rat samples.

    Anti-TREM2 (extracellular)-FITC Antibody (#ANR-018-F) is directly conjugated to fluorescein isothiocyanate (FITC). The antibody can be used in immunofluorescent applications such as direct live cell flow cytometry.

    For research purposes only, not for human use