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Anti-TRPM2-ATTO Fluor-594 Antibody

Transient receptor potential cation channel subfamily M member 2, LTrpC2, TrpC7
Cat #: ACC-043-AR
Alternative Name Transient receptor potential cation channel subfamily M member 2, LTrpC2, TrpC7
  • KO Validated
  • Lyophilized Powder yes
    Type: Polyclonal
    Host: Rabbit
    Reactivity: h, m, r
    Immunogen
    • Peptide (C)HTFQGKEWDPKKHVQE, corresponding to amino acid residues 105-120 of mouse TRPM2 (Accession Q91YD4). Intracellular, N-terminus.
    Accession (Uniprot) Number Q91YD4
    Gene ID 28240
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Rat - 15/16 amino acid residues identical; human - 14/16 amino acid residues identical.
    RRID AB_2040246.
    Purity Affinity purified on immobilized antigen.
    Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Isotype Rabbit IgG.
    Label ATTO-594. Maximum absorption 601 nm; maximum fluorescence 627 nm. The fluorescence is excited most efficiently in the 580 - 615 nm range. This label is related to the Rhodamine dyes and can be used with filters used to detect Texas Red and Alexa-594.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 50 µl double distilled water (DDW).
    Antibody concentration after reconstitution 1 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 × g 5 min).
    Standard quality control of each lot Western blot analysis (unlabeled antibody, #ACC-043), and immunohistochemistry (labeled antibody).
    Applications: ic, if, ih
    Immunohistochemistry
    • Expression of TRPM2 in rat lung
      Expression of TRPM2 in rat lung
      Immunohistochemical staining of rat lung frozen sections using Anti-TRPM2-ATTO Fluor-594 Antibody (#ACC-043-AR), (1:50). A. TRPM2 staining is specific for respiratory epithelium in the bronchi. B. Merged with bright field.
    References
    1. Fleig, A. et al. (2004) Trends. Pharmacol. Sci. 25, 633.
    2. Kraft, R. et al. (2005) Pflugers. Arch. 451, 204.
    3. Huang, C.L. (2004) J. Am. Soc. Nephrol. 15, 1690.
    4. Montell, C. (2005) Sci. STKE. 2005, re3.
    5. Du, J. et al. (2009) Proc. Natl. Acad. Sci. U.S.A. 106, 7239.
    Scientific background

    The mammalian melastatin-related transient receptor potential (TRPM) is a subfamily of the TRP family. The family was named after the first discovered member, melastatin (TRPM1) whose gene was identified in metastatic and benign melanomas1-3.

    The TRPM family consists of eight members designated as TRPM1-8 that can be further divided into four pairs: TRPM1 and TRPM3; TRPM2 and TRPM8; TRPM4 and TRPM5; and TRPM6 and TRPM71,2.

    The TRPM proteins share structural homology with other members of the TRP superfamily channels; six putative transmembrane domains, and cytoplasmic N- and C-termini. However, due to their long N- and C-termini they are also named the long TRP channel family1-3.

    The C-terminal regions of three TRPM members (TRPM2, TRPM6 and TRPM7) are occupied by enzymatic domains. Almost all the C-terminal region of TRPM2 has an enzymatic domain with sequence similarities to Nudix hydrolases (cleave mononucleotide and dinucleotide polyphosphates2,4.

    TRPM2 is a Ca2+-permeable, nonselective cation channel that is predominantly expressed in various regions of the brain, where it is preferentially localized in microglial cells, the host macrophages of the central nervous system and is also expressed in other tissues, including spleen, heart, liver, lung, and bone marrow. Several splice variants of TRPM2 have been identified5,3.

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Last update: 16/08/2020

    Anti-TRPM2 Antibody (#ACC-043) is a highly specific antibody directed against an intracellular epitope of mouse TRPM2. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize TRPM2 from mouse, rat, and human samples.

    Anti-TRPM2-ATTO Fluor-594 Antibody (#ACC-043-AR) is directly labeled with an ATTO-594 fluorescent dye. ATTO dyes are characterized by strong absorption (high extinction coefficient), high fluorescence quantum yield, and high photo-stability. The ATTO-594 fluorescent label belongs to the class of Rhodamine dyes and can be used with fluorescent equipment typically optimized to detect Texas Red and Alexa-594. Anti-TRPM2-ATTO Fluor-594 Antibody can be used for immunohistochemical applications and is specially suited to experiments requiring simultaneous labeling of different markers.

    For research purposes only, not for human use

    Applications

    Specifications

    Scientific Background

    Citations

    Citations
    KO validation citations
    1. Western blot and immunocytochemistry of mouse brain endothelial cells with #ACC-043. Tested in siRNA treated cells.
      Park, L. et al. (2014) Nat. Commun. 5, 5318.
    Immunocytochemistry citations
    1. Mouse pancreatic acinar cells (1:100).
      Fanczal, J. et al. (2020) J. Physiol. 598, 1253.
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