Anti-TRPM2 (extracellular) Antibody

Transient receptor potential cation channel subfamily M member 2, Transient receptor potential melastatin 2, LTrpC2, TrpC7
  • New
Cat #: ACC-128
Alternative Name Transient receptor potential cation channel subfamily M member 2, Transient receptor potential melastatin 2, LTrpC2, TrpC7
  • Lyophilized Powder
  • Antigen Incl.
  • Type: Polyclonal
    Host: Rabbit
    Reactivity: h, m, r
    Immunogen
    • Peptide (C)NGTDPYKPK(S)PESD, corresponding to amino acid residues 999-1012 of human TRPM2 (Accession O94759). 3rd extracellular loop.
    • Anti-TRPM2 (extracellular) Antibody
    Accession (Uniprot) Number O94759
    Gene ID 7226
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Human, mouse, rat – 13/14 amino acid residues identical.
    Purity Affinity purified on immobilized antigen.
    Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Isotype Rabbit IgG
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Negative control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
    Negative control antigen reconstitution 100 µl double distilled water (DDW).
    Negative control antigen storage after reconstitution -20°C.
    Preadsorption Control 1 µg peptide per 1 µg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: ifc, ih, wb
    May also work in: ic*, ip*, lci*
    Western blot
    • Anti-TRPM2 (extracellular) Antibody
      Western blot analysis of mouse brain lysate (lanes 1 and 3) and rat brain lysate (lanes 2 and 4):
      1, 2. Anti-TRPM2 (extracellular) Antibody (#ACC-128), (1:200).
      3, 4. Anti-TRPM2 (extracellular) Antibody, preincubated with the negative control antigen.
    • Anti-TRPM2 (extracellular) Antibody
      Western blot analysis of human THP-1 monocytic leukemia cell line lysate (lanes 1 and 3) and human HL-60 promyelocytic leukemia cell line lysate (lanes 2 and 4):
      1, 2. Anti-TRPM2 (extracellular) Antibody (#ACC-128), (1:200).
      3, 4. Anti-TRPM2 (extracellular) Antibody, preincubated with the negative control antigen.
    Immunohistochemistry
    • Anti-TRPM2 (extracellular) Antibody
      Expression of TRPM2 in rat substantia nigra
      Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-TRPM2 (extracellular) Antibody (#ACC-128), followed by goat anti-rabbit-AlexaFluor-488. TRPM2 immunoreactivity (green) appears in neurons in the substantia nigra pars compacta (SNC), (arrows). SNR=substantia nigra pars reticulata. Cell nuclei are stained with DAPI (blue).
    Indirect flow cytometry
    • Anti-TRPM2 (extracellular) Antibody
      Cell surface detection of TRPM2 in live intact human THP-1 monocytic leukemia cells:
      ___ Cells.
      ___ Cells + goat-anti-rabbit-FITC.
      ___ Cells + Anti-TRPM2 (extracellular) Antibody (#ACC-128), (2.5 µg) + goat-anti-rabbit-FITC.
    References
    1. Fleig, A. et al. (2004) Trends. Pharmacol. Sci. 25, 633.
    2. Kraft, R. et al. (2005) Pflugers. Arch. 451, 204.
    3. Huang, C.L. (2004) J. Am. Soc.Nephrol15, 1690.
    4. Montell, C. (2005) Sci. STKE. 2005, re3.
    5. Du, J. et al. (2009) Proc. Natl. Acad. Sci. U.S.A. 106, 7239.
    Scientific background

    The mammalian melastatin-related transient receptor potential (TRPM) is a subfamily of the TRP family. The family was named after the first discovered member, melastatin (TRPM1) whose gene was identified in metastatic and benign melanomas1-3

    The TRPM family consists of eight members: TRPM1-8 that can be further divided into four pairs: TRPM1 and TRPM3; TRPM2 and TRPM8; TRPM4 and TRPM5; and TRPM6 and TRPM71,2.

    The TRPM proteins share structural homology with other members of the TRP superfamily channels; six putative transmembrane domains, and cytoplasmic N- and C-termini. However, due to their long N- and C-termini they are also named the long TRP channel family1-3

    The C-terminal regions of three TRPM members (TRPM2, TRPM6 and TRPM7) are occupied by enzymatic domains.  Almost all the C-terminal region of TRPM2 has an enzymatic domain with sequence similarities to Nudix hydrolases (cleave mononucleotide and dinucleotide polyphosphates2,4.

    TRPM2 (LTRpC2, TrpC7) is a Ca2+-permeable, nonselective cation channel that is predominantly expressed in various regions of the brain, where it is preferentially localized in microglial cells, the host macrophages of the central nervous system and is also expressed in other tissues, including spleen, heart, liver, lung, and bone marrow. Several splice variants of TRPM2 have been identified5,3.

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat

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    Last update: 11/02/2020

    Anti-TRPM2 (extracellular) Antibody (#ACC-128) is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot, immunohistochemistry, and live cell flow cytometry applications. It has been designed to recognize TRPM2 from mouse, rat, and human samples.

    For research purposes only, not for human use

    Applications

    Specifications

    Scientific Background

    Citations

    Citations
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