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Anti-TRPM2 Antibody

Transient receptor potential cation channel subfamily M member 2, LTrpC2, TrpC7

Cat #: ACC-043
Alternative Name Transient receptor potential cation channel subfamily M member 2, LTrpC2, TrpC7
  • KO Validated
  • Lyophilized Powder yes
    Type: Polyclonal
    Host: Rabbit
    Reactivity: h, m, r
    Immunogen
    • Peptide (C)HTFQGKEWDPKKHVQE, corresponding to amino acid residues 105-120 of mouse TRPM2 (Accession Q91YD4). Intracellular, N-terminus.
    Accession (Uniprot) Number Q91YD4
    Gene ID 28240
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Rat - 15/16 amino acid residues identical; human - 14/16 amino acid residues identical.
    RRID AB_2040248.
    Purity Affinity purified on immobilized antigen.
    Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Isotype Rabbit IgG.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 25 μl, 50 μl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.8 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Standard quality control of each lot Western blot analysis.
    Applications: ic, if, ih, wb
    May also work in: ifc*, ip*
    Western blot
    • Western blot analysis of rat lung membrane (lanes 1 and 4), rat brain membrane (lanes 2 and 5) and human K562 cells (lanes 3 and 6) lysates:
      Western blot analysis of rat lung membrane (lanes 1 and 4), rat brain membrane (lanes 2 and 5) and human K562 cells (lanes 3 and 6) lysates:
      1-3. Anti-TRPM2 Antibody (#ACC-043), (1:200).
      4-6. Anti-TRPM2 Antibody, preincubated with TRPM2 Blocking Peptide (#BLP-CC043).
    Immunohistochemistry
    • Expression of TRPM2 in rat lung
      Expression of TRPM2 in rat lung
      Immunohistochemical staining of rat lung paraffin embedded sections using Anti-TRPM2 Antibody (#ACC-043), (1:100). TRPM2 is expressed in the respiratory epithelium (black arrows). In addition, staining is also present in smooth muscle, both bronchiolar (arrowheads) and vascular (red arrows). Hematoxilin is used as the counterstain.
    Immunocytochemistry
    • Mouse brain endothelial cells (1:200), (Park, L. et al. (2014) Nat. Commun. 5, 5318.).
    References
    1. Fleig, A. et al. (2004) Trends. Pharmacol. Sci. 25, 633.
    2. Kraft, R. et al. (2005) Pflugers. Arch. 451, 204.
    3. Huang, C.L. (2004) J. Am. Soc. Nephrol. 15, 1690.
    4. Montell, C. (2005) Sci. STKE. 2005, re3.
    5. Du, J. et al. (2009) Proc. Natl. Acad. Sci. U.S.A. 106, 7239.
    Scientific background

    The mammalian melastatin-related transient receptor potential (TRPM) is a subfamily of the TRP family. The family was named after the first discovered member, melastatin (TRPM1) whose gene was identified in metastatic and benign melanomas1-3

    The TRPM family consists of eight members designated as TRPM1-8 that can be further divided into four pairs: TRPM1 and TRPM3; TRPM2 and TRPM8; TRPM4 and TRPM5; and TRPM6 and TRPM71,2.

    The TRPM proteins share structural homology with other members of the TRP superfamily channels; six putative transmembrane domains, and cytoplasmic N- and C-termini. However, due to their long N- and C-termini they are also named the long TRP channel family1-3

    The C-terminal regions of three TRPM members (TRPM2, TRPM6 and TRPM7) are occupied by enzymatic domains.  Almost all the C-terminal region of TRPM2 has an enzymatic domain with sequence similarities to Nudix hydrolases (cleave mononucleotide and dinucleotide polyphosphates2,4.

    TRPM2 (LTRpC2, TrpC7) is a Ca2+-permeable, nonselective cation channel that is predominantly expressed in various regions of the brain, where it is preferentially localized in microglial cells, the host macrophages of the central nervous system and is also expressed in other tissues, including spleen, heart, liver, lung, and bone marrow. Several splice variants of TRPM2 have been identified5,3.

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Last update: 24/01/2021

    Anti-TRPM2 Antibody (#ACC-043) is a highly specific antibody directed against an intracellular epitope of mouse TRPM2. The antibody can be used in western blot, immunocytochemistry, and immunohistochemistry applications. It has been designed to recognize TRPM2 from mouse, rat, and human samples.

    For research purposes only, not for human use

    Applications

    Specifications

    Scientific Background

    Citations

    Citations
    KO validation citations
    1. Western blot and immunocytochemistry of mouse brain endothelial cells. Tested in siRNA treated cells.
      Park, L. et al. (2014) Nat. Commun. 5, 5318.
    Western blot citations
    1. Human ARPE-19 retinal pigment epithelial cell lysate (1:200).
      Melendez Garcia, R. et al. (2016) Ebiomedicine 7, 35.
    2. Mouse brain endothelial cell lysate (1:200).
      Park, L. et al. (2014) Nat. Commun. 5, 5318.
    Immunohistochemistry citations
    1. Human pulpal tissue sections (1:100).
      Rowland, K.C. et al. (2007) J. Endod. 33, 245.
    Immunocytochemistry citations
    1. Mouse brain endothelial cells (1:200).
      Park, L. et al. (2014) Nat. Commun. 5, 5318.
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