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Anti-TRPM6 (extracellular)-FITC Antibody

Transient receptor potential cation channel subfamily M member 6, Channel kinase 2, CHAK2, Melastatin-related TRP cation channel 6

Cat #: ACC-046-F
Alternative Name Transient receptor potential cation channel subfamily M member 6, Channel kinase 2, CHAK2, Melastatin-related TRP cation channel 6
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h, m, r
Immunogen
  • Peptide CVKDYDLERGPDEK, corresponding to amino acid residues 802-815 of mouse TRPM6 (Accession Q8CIR4). 1st extracellular loop.
Accession (Uniprot) Number Q8CIR4
Gene ID 225997
Homology Rat - 13/14 amino acid residues identical; human - 11/14 amino acid residues identical.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG
Label Fluorescein isothiocyanate (FITC)
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 15 µl or 50 µl double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 1 mg/ml
Storage after reconstitution The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 × g 5 min).
Standard quality control of each lot Western blot analysis (unlabeled antibody, #ACC-046), and direct flow cytometry (labeled antibody).
Applications: fc, lci
Direct flow cytometry
  • Cell surface detection of TRPM6 by direct flow cytometry in live intact mouse J774 macrophage cells:
    Cell surface detection of TRPM6 by direct flow cytometry in live intact mouse J774 macrophage cells:
    ___ Cells.
    ___ Cells + Rabbit IgG Isotype Control-FITC (#RIC-001-F).
    ___ Cells + Anti-TRPM6 (extracellular)-FITC Antibody (#ACC-046-F), (2.5µg).
  • Cell surface detection of TRPM6 by direct flow cytometry in live intact human Jurkat T-cell leukemia cells:
    Cell surface detection of TRPM6 by direct flow cytometry in live intact human Jurkat T-cell leukemia cells:
    ___ Cells.
    ___ Cells + Rabbit IgG Isotype Control-FITC (#RIC-001-F).
    ___ Cells + Anti-TRPM6 (extracellular)-FITC Antibody (#ACC-046-F), (5µg).
  • Cell surface detection of TRPM6 by direct flow cytometry in live intact human THP-1 monocytic leukemia cells:
    Cell surface detection of TRPM6 by direct flow cytometry in live intact human THP-1 monocytic leukemia cells:
    ___ Cells.
    ___ Cells + Rabbit IgG Isotype Control-FITC (#RIC-001-F).
    ___ Cells + Anti-TRPM6 (extracellular)-FITC Antibody (#ACC-046-F), (5µg).
References
  1. Ramsey, S.I. et al. (2006) Annu. Rev. Physiol. 68, 619.
  2. Pedersen, S.F. et al. (2005) Cell. Calcium. 38, 233.
  3. Montell, C. (2005) Sci. STKE 2005, re3.
  4. Hilgemann, D.W. et al. (2001) Sci. STKE 2001, re19.
  5. Schmitz, C. et al. (2005) J. Biol. Chem. 280, 37763.
Scientific background

TRP channels are a large family (about 28 genes) of plasma membrane, non-selective cationic channels that are either specifically or ubiquitously expressed in excitable and non-excitable cells.1 The TRP channels have putative six-transmembrane domains (TM) with a pore domain between the fifth and the six TM, and all assemble as tetramers. Both the N- and the C-terminus of all TRPs are intracellular.3

According to IUPHAR the TRP family comprises of three main subfamilies on the basis of sequence homology; TRPC (canonical), TRPV (vanilloid) and TRPM (melastatin). To date, three extra subfamilies are also considered to belong to the TRP family; the TRPA, TRPML, and the TRPP.1-4

The TRPM subfamily consists of eight members, TRPM1 to TRPM8, which also can be further subdivided into four subgroups based on their sequence homology: (1)TRPM1 and TRPM3 (2) TRPM6 and TRPM7 (3) TRPM4 and TRPM5 (4) TRPM2 and TRPM8.5

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 31/07/2022

Anti-TRPM6 (extracellular) Antibody (#ACC-046) is a highly specific antibody directed against an extracellular epitope of the mouse protein. The antibody can be used in western blot, immunohistochemistry and live cell flow cytometry applications. It has been designed to recognize TRPM6 from mouse, rat and human samples.

Anti-TRPM6 (extracellular)-FITC Antibody (#ACC-046-F) is directly conjugated to fluorescein isothiocyanate (FITC) fluorophore. This conjugated antibody has been developed to be used in immunofluorescent applications such as direct flow cytometry and live cell imaging. It has been designed to recognize TRPM6 from mouse, rat and human samples.

For research purposes only, not for human use
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