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Anti-TRPM7 Antibody

LTrpC7, TRP-PLIK, Channel-kinase 1, CHAK1, ALSPDC

Cat #: ACC-047
Alternative Name LTrpC7, TRP-PLIK, Channel-kinase 1, CHAK1, ALSPDC
  • KO Validated
  • Lyophilized Powder yes
    Type: Polyclonal
    Host: Rabbit
    Reactivity: h, m, r
    Immunogen
    • Peptide CKRRKKDKTSDGPKLFLTEE, corresponding to amino acid residues 1146-1165 of human TRPM7 (Accession Q96QT4). Intracellular, C-terminus.
    Accession (Uniprot) Number Q96QT4
    Gene ID 54822
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Rat, mouse - identical.
    RRID AB_2039806.
    Purity Affinity purified on immobilized antigen.
    Form Lyophilized powder. Reconstituted antibody contains Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Isotype Rabbit IgG.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.8 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Standard quality control of each lot Western blot analysis.
    Applications: ic, if, ih, wb
    May also work in: ifc*, ip*
    Western blot
    • Western blot analysis of rat GH3 pituitary tumor cell line (lanes 1 and 2) and mouse brain (lanes 3 and 4) lysates:
      Western blot analysis of rat GH3 pituitary tumor cell line (lanes 1 and 2) and mouse brain (lanes 3 and 4) lysates:
      1,3. Anti-TRPM7 Antibody (#ACC-047), (1:200).
      2,4. Anti-TRPM7 Antibody, preincubated with TRPM7 Blocking Peptide (#BLP-CC047).
    Immunohistochemistry
    • Expression of TRPM7 in rat pituitary gland
      Expression of TRPM7 in rat pituitary gland
      Immunohistochemical staining of rat hypophysis paraffin embedded transversal sections using Anti-TRPM7 Antibody (#ACC-047), (1:50). TRPM7 is stained in both the posterior part or neurohypophysis (N) and the anterior part or adenohypophysis (A). Note that stain is highly specific for andocrine cells in the adenohypophysis. Color reaction was obtained with SuperPicture HRP-conjugated polymer (Zymed) followed by DAB. Hematoxilin is used as the Counterstain.
    Immunocytochemistry
    • Expression of TRPM7 in GH3 cell lines
      Expression of TRPM7 in GH3 cell lines
      Immunocytochemical staining of GH3 cells with Anti-TRPM7 Antibody (#ACC-047), (1:100), followed by goat anti-rabbit-AlexaFluor-550 secondary antibody (A). B. Nuclear staining of GH3 cells with the cell-permeable dye Hoechst 33342. C. Live intact GH3 cells.
    References
    1. Montell, C. et al. (2002) Mol. Cell. 9, 229.
    2. Clapham, D.E. (2003) Nature 426, 517.
    3. Moran, M.M. et al. (2004) Curr. Opin. Neurobiol. 14, 362.
    4. Clapham, D.E. et al. (2003) Pharmacol. Rev. 55, 591.
    5. Harteneck, C. (2005) Naunyn. Schmiedebergs. Arch. Pharmacol. 371, 307.
    6. Schmitz, C. et al. (2005) J. Biol. Chem. 280, 37763.
    7. Aarts, M.M. et al. (2005) Neuroscientist 11, 116.
    8. Aarts, M.M. et al. (2005) Pflugers. Arch. 451, 243.
    Scientific background

    TRP channels are a large family (about 28 genes) of plasma membrane, non-selective cationic channels that are either specifically or ubiquitously expressed in excitable and non-excitable cells.1 The TRP channels have putative six-transmembrane domains (TM) with a pore domain between the fifth and the six TM, and all assemble as tetramers. Both the N- and the C-terminus of all TRPs are intracellular.3

    According to IUPHAR the TRP family comprises of three main subfamilies on the basis of sequence homology; TRPC (canonical), TRPV (vanilloid) and TRPM (melastatin). To date, three extra subfamilies are also considered to belong to the TRP family; the TRPA, TRPML, and the TRPP.1-4

    The TRPM subfamily consists of eight members, TRPM1 to TRPM8, which also can be further subdivided into four subgroups based on their sequence homology: (1)TRPM1 and TRPM3 (2) TRPM6 and TRPM7 (3) TRPM4 and TRPM5 (4) TRPM2 and TRPM8.5

    TRPM7 and TRPM6 are involved in Mg2+ homeostasis and are unique among the TRP family members, possessing a functional kinase domain at their C-terminus.6,7 Although the kinase is not necessary to the function of the channel it may have a role in modulating the activation of channel 7.Recent work demonstrated that TRPM7 is a critical mediator of anoxic cell death.7,8

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Image & Title: Anti-TRPM7 Antibody
    Expression of TRPM7 in human cardiomyocytes.Immunocytochemical staining of human cardiomyocytes using Anti-TRPM7 Antibody (#ACC-047). TRPM7 expression is shown in green. Phalloidin (red) is used to stain actin filaments and Hoechst 33342 (blue) is used to stain nuclei.Adapted from Macianskiene, R. et al. (2017) PLoS ONE 12, e0170923. with permission of PLoS.
    Last update: 02/08/2022

    Alomone Labs is pleased to offer a highly specific antibody directed against an intracellular epitope of human TRPM7. Anti-TRPM7 Antibody (#ACC-047) can be used in western blot, immunocytochemistry, and immunohistochemistry applications. It has been designed to recognize TRPM7 from human, rat, and mouse samples.

    For research purposes only, not for human use

    Applications

    Citations

    Citations
    KO validation citations
    1. Immunohistochemistry of mouse brain lysates. Tested in TRPM7 conditional knockout mice.
      Zhang, P. et al. (2022) Cells 11, 1178.
    Immunocytochemistry citations
    1. Human cardiomyocytes.
      Macianskiene, R. et al. (2017) PLoS ONE 12, e0170923.
    2. Mouse NIE-115 neuroblastoma transfected cells (1:500).
      Visser, D. et al. (2013) Cell. Calcium. 54, 404.
    More product citations
    1. Zhang, Y. et al. (2012) Neurol. Sci. 33, 1125.
    2. Akita, T. et al. (2011) J. Physiol. 589, 3909.
    3. Zhang, J. et al. (2011) J. Biol. Chem. 286, 20194.
    4. Cheng, H. et al. (2010) Stem. Cells. Dev. 19, 1393.

    Specifications

    Scientific Background

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