Name: Anti-TRPM8 (extracellular) Antibody
Brand: Alomone Labs
SKU: ACC-049

Cat #: ACC-049

Alternative Name TRPP8, LTrpC6, Transient receptor potential cation channel subfamily M member 8

Lyophilized Powder yes

Type: Polyclonal

Host: Rabbit

Reactivity: h, m, r

Immunogen

Peptide SDVDGTTYDFAHC, corresponding to amino acid residues 917-929 of human TRPM8 (Accession Q7Z2W7). 3^rdextracellular loop.

Accession (Uniprot) Number Q7Z2W7

Gene ID 79054

Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.

Homology Canis - identical; bovine - 12/13 amino acid residues identical; rat, mouse - 11/13 amino acid residues identical.

RRID AB_2040254.

Purity Affinity purified on immobilized antigen.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN₃.

Isotype Rabbit IgG.

Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.

Antibody concentration after reconstitution 0.8 mg/ml.

Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Standard quality control of each lot Western blot analysis.

Applications: ic, if, ih, ip, lci, n, wb

May also work in: ifc*

Western blot

LNCaP and DU145, human prostate carcinoma cell lines, mouse-TRPM8 transfected HEK-293 cells (1:200). It is recommended to add 0.5% Tween-20 to the antibody solution.
Western blot analysis of prostate carcinoma cell lines DU145 (lanes 1 and 3), Human LNCaP prostate carcinoma (lanes 2 and 4) and mouse-TRPM8 transfected HEK-293 (lanes 5 and 6) cell lysates:
1,2,5. Anti-TRPM8 (extracellular) Antibody (#ACC-049), (1:200).
3,4,6. Anti-TRPM8 (extracellular) Antibody, preincubated with TRPM8 (extracellular) Blocking Peptide (#BLP-CC049).

Immunoprecipitation

Transfected HEK-293 cells (Zeevi, D.A. et al. (2010) J. Cell Sci. 123, 3112.).

Immunohistochemistry

Rat dorsal root ganglia (DRG) frozen section (1:100).

Immunocytochemistry

Human prostate cancer DU145 cells (1:50) (Wang, Y. et al. (2012) Pathol. Oncol. Res. 18, 903.).

Neutralization

Human, mouse and rat TRPM8 expressed in CHO cells, and rat DRGs (Miller, S. et al. (2014) PLoS ONE 9, e107151).

Live cell imaging / Immunocytochemistry

Human live LNCaP cells (1:100). Rat live DRGs (1:500).

Scientific background

The mammalian melastatin-related transient receptor potential (TRPM) is a subfamily of the TRP family. The family was named after the first member, melastatin (TRPM1), whose gene was identified in metastatic and benign melanomas.¹ The TRPM proteins share structural homology with other members of the TRP superfamily channels; six putative transmembrane domains, and cytoplasmic N-terminus and C-terminus. However, due to their long N-terminus and C-terminus they were also named the long TRP channel family.¹

The TRPM family consists of eight members designated as TRPM1-8 that can be further divided into four pairs: TRPM1 and TRPM3; TRPM2 and TRPM8; TRPM4 and TRPM5; and TRPM6 and TRPM7.¹

The TRPM8 channel is the cold and menthol receptor activated by either cold temperatures(~28°C) or menthol.²

TRPM8 is expressed in dorsal root ganglia (DRGs) where about 5%-10% of the small diameter DRG neurons express the channel.^3,4 In DRGs, TRPM8 expressing neurons do not express the TRPV1 channel.⁵ Overexpression of TRPM8 was found in prostate cancer cells. However, the physiological and pathological roles that these cells play is still elusive.⁶ In prostate, it was suggested that TRPM8 might play a possible role in the progression of cancer to the metastatic stage.⁶

Lyophilized Powder

Image & Title:

Expression of TRPM8 in rat tail artery astrocytes.Immunocytochemical staining of rat tail artery astrocytes using Anti-TRPM8 (extracellular) Antibody (#ACC-049). TRPM8 staining is shown in green, nuclei are in blue. Omission of the antibody (lower panel) abolishes TRPM8 staining.Adapted from Johnson, C.D. et al. (2009) Am. J. Physiol. 296, H1868. with permission of the American Physiological Society.

Last update: 08/01/2023

For research purposes only, not for human use

Specifications

Citations

Applications

Scientific Background

Shipping and Ordering information

Control Product

TRPM8 (extracellular) Blocking Peptide (#BLP-CC049) is the original antigen used for immunization during Anti-TRPM8 (extracellular) Antibody (#ACC-049) generation. The blocking peptide binds and ‘blocks’ Anti-TRPM8 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.

TRPM8 (extracellular) Blocking Peptide (#BLP-CC049)

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Certificate of Analysis

Anti-TRPM8 (extracellular) Antibody

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Specifications

Citations

Applications

Scientific Background

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