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Anti-TRPV2 (VRL1) (extracellular) Antibody

Transient receptor potential cation channel subfamily V member 2, Vanilloid receptor-like protein 1, VRL-1, Osm-9-like TRP channel 2, OTRPC2, Stretch-activated channel 2B, SAC2B
Cat #: ACC-039
Alternative Name Transient receptor potential cation channel subfamily V member 2, Vanilloid receptor-like protein 1, VRL-1, Osm-9-like TRP channel 2, OTRPC2, Stretch-activated channel 2B, SAC2B
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: m, r
Immunogen
  • Peptide (C)HQPSLDQPAIPSSKAT, corresponding to amino acid residues 413-428 of rat TRPV2 (Accession Q9WUD2). 1st extracellular loop.
Accession (Uniprot) Number Q9WUD2
Gene ID 29465
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Mouse - 15/16 amino acid residues identical.
RRID AB_2040268.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 μl, 50 μl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.6 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: ic, if, ifc, ih, ip, lci, wb
Western blot
  • Western blot analysis of ND7/23 cell line membrane (lanes 1 and 3), RBL lysates (lanes 2 and 4) and rat brain membrane (lanes 5 6):
    Western blot analysis of ND7/23 cell line membrane (lanes 1 and 3), RBL lysates (lanes 2 and 4) and rat brain membrane (lanes 5 6):
    1,2,5. Anti-TRPV2 (VRL1) (extracellular) Antibody (#ACC-039), (1:200).
    3,4,6. Anti-TRPV2 (VRL1) (extracellular) Antibody, preincubated with TRPV2/VRL1 (extracellular) Blocking Peptide (#BLP-CC039).
Immunoprecipitation
  • Immunoprecipitation of rat basophilic leukemia (RBL) cell lysate:
    Immunoprecipitation of rat basophilic leukemia (RBL) cell lysate:
    1. RBL lysate.
    2. Lysate immunoprecipitated with Anti-TRPV2 (VRL1) (extracellular) Antibody (#ACC-039), (6 µg).
    3. Lysate immunoprecipitated with pre-immune rabbit serum. The upper arrow indicates TRPV2 while the lower arrow indicates the IgG heavy chain. Western blot analysis was performed with Anti-TRPV2 (VRL1) (extracellular) Antibody.
Immunohistochemistry
  • Expression of TRPV2 in mouse DRG
    Expression of TRPV2 in mouse DRG
    Immunohistochemical staining of TRPV2 in mouse dorsal root ganglion (DRG) using Anti-TRPV2 (VRL1) (extracellular) Antibody (#ACC-039).  A. TRPV2 (green) appears in patches along the perimeter of the DRG (arrows). B. Neurons containing neurofilament 200 (red) are scattered in the DRG, also in patches (arrows). C. A merge of the two panels shows that the spatial distribution of neurofilament 200 and TRPV2 expression overlaps. However, DRGs showing robust expression of neurofilament 200 do not contain TRPV2.
Indirect flow cytometry
  • Cell surface detection of TRPV2 in intact living RBL cells:
    Cell surface detection of TRPV2 in intact living RBL cells:
    ___ Unstained cells.
    ___ Cells + Anti-TRPV2 (VRL1) (extracellular) Antibody (#ACC-039).
Live cell imaging / Immunocytochemistry
  • Expression of TRPV2 in RBL cells
    Expression of TRPV2 in RBL cells
    Cell surface detection of TRPV2 in intact living rat basophilic leukemia (RBL) cells with Anti-TRPV2 (VRL1) (extracellular) Antibody (#ACC-039), (1:100), followed by goat anti-rabbit-AlexaFluor-550 secondary antibody (red), (x100).
References
  1. Montell, C. et al. (2002) Mol. Cell 9, 229.
  2. Clapham, D.E. (2003) Nature 426, 517
  3. Moran, M.M. et al. (2004) Curr. Opin. Neurobiol. 14, 362.
  4. Clapham, D.E. et al. (2003) Pharmacol. Rev. 55, 591.
  5. Gunthorpe, M.J. et al. (2002) Trends Pharmacol. Sci. 23, 183.
  6. Tominaga, M. and Caterina, M.J. et al. (2004) J. Neurobiol. 61, 3.
  7. Voets, T. et al. (2004) Nature 430, 748.
  8. Pedersen, S.F. et al. (2005) Cell Calcium 38, 233.
  9. Muraki, K. et al. (2003) Circ. Res. 93, 829.
  10. Shimosato, G. et al. (2005) Pain 119, 225.
  11. Saunders, C.I. et al. (2007) Mol. Immunol. 44, 1429.
  12. Caterina, M.J. et al. (1999) Nature 398, 436.
Scientific background

TRP channels are a large family (about 28 genes) of plasma membrane, non-selective cationic channels that are either specifically or ubiquitously expressed in excitable and non-excitable cells.1 The TRP channels have six putative transmembrane domains (TM) with a pore domain between the fifth and the sixth TM, and all assemble as tetramers. Both the N- and the C-termini of all TRPs are intracellular3.

According to IUPHAR, the TRP family is comprised of numerous subfamilies on the basis of sequence homology; TRPC, TRPM, TRPV, TRPA, TRPML, and TRPP1-4. The TRPV subfamily consists of six members, TRPV1-65.

Four members of the TRPV family have been described as thermosensitive ion channels (TRPV1 to TRPV4). Each channel exhibits distinct thermal activation thresholds ranging from noxious cold (<17°C) to noxious heat (>52°C) 6,7. Although it shares around 50% homology with TRPV1, TRPV2 is not activated by capsaicin nor by protons. It has a high temperature threshold of ~52°C and is considered to play an essential role in the perception of high-intensity noxious heat stimulation8-10. TRPV2 is also considered to be a stretch-activated channel and to play a role in skeletal and cardiac muscle degeneration and pain pathway8. The TRPV2 channel is expressed in DRG neurons, different brain regions and non-neuronal tissues such as the spleen, lung and intestine and in components of the immune system5,11,12.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Image & Title: Anti-TRPV2 (VRL1) (extracellular) AntibodyExpression of TRPV2 in mouse cardiomyocytes.Immunocytochemical staining of mouse cardiomyocytes. Staining of cells using Anti-TRPV2 (VRL1) (extracellular) Antibody (#ACC-039) (green) shows that TRPV2 is translocated to the sarcolemma along the T-tubules in cardiomyocytes from DMD mice (lower left panel).Adapted from Lorin, C. et al. (2015) with permission of European Society of Cardiology.
Last update: 16/08/2020

Anti-TRPV2 (VRL1) (extracellular) Antibody (#ACC-039) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot, immunoprecipitation, immunohistochemistry, live cell imaging, and indirect flow cytometry applications. It has been designed to recognize TRPV2 from mouse and rat samples.

For research purposes only, not for human use

Applications

Specifications

Scientific Background

Citations

Citations
Western blot citations
  1. Rat astrocyte lysates.
    Zhang, H. et al. (2016) Eur. J. Neurosci. 44, 2493.
  2. Mouse cardiomyocyte lysates (1:200).
    Lorin, C. et al. (2015) Cardiovasc. Res. 106, 153.
Immunohistochemistry citations
  1. Mouse heart sections.
    Aguettaz, E. et al. (2016) Cell Calcium 59, 145.
  2. Rat brain sections.
    Zhang, H. et al. (2016) Eur. J. Neurosci. 44, 2493.
Immunocytochemistry citations
  1. Rat astrocytes.
    Zhang, H. et al. (2016) Eur. J. Neurosci. 44, 2493.
  2. Mouse cardiomyocytes (1:100).
    Lorin, C. et al. (2015) Cardiovasc. Res. 106, 153.
Immunofluorescence citations
  1. Mouse heart sections.
    Aguettaz, E. et al. (2016) Cell Calcium 59, 145.
Neutralization citations
  1. Rat retinal vascular smooth muscle cells.
    McGahon, M.K. et al. (2016) Invest. Ophtalmol. Vis. Sci. 57, 5637.
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