Anti-TRPV3 (extracellular)-ATTO-633 Antibody

Transient receptor potential cation channel subfamily V member 3, Vanilloid receptor-like 3, VRL3
    Cat #: ACC-033-FR
    Alternative Name Transient receptor potential cation channel subfamily V member 3, Vanilloid receptor-like 3, VRL3
  • Lyophilized Powder
  • Antigen Incl.
  • Type: Polyclonal
    Host: Rabbit
    Reactivity: h, m, r
    Immunogen
    • Peptide (C)REEEAIPHPLALTHK, corresponding to amino acid residues 464-478 of human TRPV3 (Accession Q8NET8). 1st extracellular loop.
    • Anti-TRPV3 (extracellular)-ATTO-633 Antibody
    Accession (Uniprot) Number Q8NET8
    Gene ID 162514
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Rat - 13/15 amino acid residues identical; mouse - 12/15 amino acid residues identical.
    RRID AB_2340899.
    Purity Affinity purified on immobilized antigen.
    Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Isotype Rabbit IgG.
    Label ATTO-633. Maximum absorption 629 nm; maximum fluorescence 657 nm. The fluorescence is excited most efficiently in the 610 - 645 nm range. This label is analogous to the dyes Alexa 647, Alexa 633 and Cy5 and can be used for direct flow cytometry (FACS) using the He:Ne laser.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 50 µl double distilled water (DDW).
    Antibody concentration after reconstitution 1 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 × g 5 min).
    Negative control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
    Negative control antigen reconstitution 100 µl double distilled water (DDW).
    Negative control antigen storage after reconstitution -20°C.
    Preadsorption Control 1 µg peptide per 1 µg antibody.
    Standard quality control of each lot Western blot analysis (unlabeled antibody, #ACC-033), and immunohistochemistry (labeled antibody).
    Applications: ic, if, ih, lci
    Immunohistochemistry
    • Anti-TRPV3 (extracellular)-ATTO-633 Antibody
      Expression of TRPV3 in rat DRG
      Immunohistochemical staining of rat dorsal root ganglion (DRG) using Anti-TRPV3 (extracellular)-ATTO-633 Antibody (#ACC-033-FR). A. TRPV3 staining (purple) is detected in DRG neurons. B. Nuclear staining using DAPI as the counterstain (Blue). C. Merge images of A and B.
    Live cell imaging / Immunocytochemistry
    • Anti-TRPV3 (extracellular)-ATTO-633 Antibody
      Expression of TRPV3 in rat PC12 cells
      Cell surface detection of TRPV3 in intact living rat pheochromocytoma (PC12) cells. A. Extracellular staining of cells using Anti-TRPV3 (extracellular)-ATTO-633 Antibody (#ACC-033-FR), (1:25), (purple). B. Merge of A with live view of the cells.
    References
    1. Montell, C. et al. (2002) Mol. Cell. 9, 229.
    2. Clapham, D.E. (2003) Nature 426, 517.
    3. Moran, M.M. et al. (2004) Curr. Opin. Neurobiol. 14, 362.
    4. Clapham, D.E. et al. (2003) Pharmacol. Rev. 55, 591.
    5. Gunthorpe, M.J. et al. (2002) Trends Pharmacol. Sci. 23, 183.
    6. Tominaga, M. and Caterina, M.J. (2004) J. Neurobiol. 61, 3.
    7. Voets, T. et al. (2004) Nature 430, 748.
    8. Xiao, R. et al. (2008) J. Biol. Chem. 283, 6162.
    Scientific background

    TRP channels are a large family (about 28 genes) of plasma membrane, non-selective cationic channels that are either specifically or ubiquitously expressed in excitable and non-excitable cells.1 The TRP channels have six putative transmembrane domains (TM) with a pore domain between the fifth and the sixth TM, and all assemble as tetramers. Both the N- and the C-terminus of all TRPs are intracellular.3

    According to IUPHAR, the TRP family is composed of three main subfamilies on the basis of sequence homology; TRPC, TRPM and TRPV (to date, three additional subfamilies are also considered to belong to the TRP family: the TRPA, TRPML, and TRPP).1-4 The TRPV subfamily consists of six members, TRPV1-6.5

    Four members of the TRPV family have been described as a thermosensitive ion channels (TRPV1 to TRPV4). Each channel exhibits distinct thermal activation thresholds ranging from noxious cold (<17°C) to noxious heat (>52°C).6,7

    TRPV3 is highly expressed in skin keratinocytes. It forms a nonselective cation channel that is activated by hot temperatures in the innocuous and noxious range. The channel is also expressed in oral and nasal cavities where it has been postulated to play a role in flavor sensation. TRPV3 is also implicated as a molecular target of some allergens and skin sensitizers.8

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Last update: 10/02/2020

    Anti-TRPV3 (extracellular) Antibody (#ACC-033) is a highly specific antibody directed against an extracellular epitope of human TRPV3. The antibody can be used for western blot, immunohistochemical and immunocytochemical applications. As the antibody recognizes an extracellular epitope, it can be used as a tool to detect TRPV3 in live cells. The antibody has been designed to recognize TRPV3 from human, rat and mouse samples.

    Anti-TRPV3 (extracellular)-ATTO-633 Antibody (#ACC-033-FR) is directly labeled with an ATTO-633 fluorescent dye. ATTO dyes are characterized by strong absorption (high extinction coefficient), high fluorescence quantum yield, and high photo-stability. ATTO 633 has a maximum absorption at 629 nm and a maximum fluorescence at 657 nm. The fluorescence is excited most efficiently in the range 610 to 645 nm. This label is analogous to the well-known dyes Alexa 647, Alexa 633 and Cy5. Anti-TRPV3 (extracellular)-ATTO-633 Antibody has been tested in immunohistochemical staining and is especially suited for experiments requiring simultaneous labeling of different markers.

    For research purposes only, not for human use
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