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Anti-TRPV3 (extracellular) Antibody

Transient receptor potential cation channel subfamily V member 3, Vanilloid receptor-like 3, VRL3
Cat #: ACC-033
Alternative Name Transient receptor potential cation channel subfamily V member 3, Vanilloid receptor-like 3, VRL3
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h, m, r
Immunogen
  • Peptide (C)REEEAIPHPLALTHK, corresponding to amino acid residues 464-478 of human TRPV3 (Accession Q8NET8). 1st extracellular loop.
Accession (Uniprot) Number Q8NET8
Gene ID 162514
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Rat - 13/15 amino acid residues identical; mouse - 12/15 amino acid residues identical.
RRID AB_2040261.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 μl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.85 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: ic, if, ih, lci, wb
May also work in: ifc*, ip*
Western blot
  • Western blot analysis of rat DRG (lanes 1 and 2) and brain (lanes 3 and 4) lysates:
    Western blot analysis of rat DRG (lanes 1 and 2) and brain (lanes 3 and 4) lysates:
    1,3. Anti-TRPV3 (extracellular) Antibody (#ACC-033), (1:200).
    2,4. Anti-TRPV3 (extracellular) Antibody, preincubated with TRPV3 (extracellular) Blocking Peptide (#BLP-CC033).
Immunohistochemistry
  • Expression of TRPV3 in mouse spinal cord
    Expression of TRPV3 in mouse spinal cord
    Immunohistochemical staining of mouse spinal cord using Anti-TRPV3 (extracellular) Antibody (#ACC-033). A. TRPV3 (green) appears in neurons (vertical arrows) in the ventral horn of the mouse spinal cord. B. Motor neurons were stained with goat anti-choline-acetyltransferase (red). In some motor neurons (A) TRPV3 was more intense (vertical arrow) whereas in others it was weak (horizontal arrows). C. Merged images of panels A and B. The inset in C magnifies one large motor neuron (vertical arrowhead). Note the punctate pattern of TRPV3 on the surface of the soma.
Live cell imaging / Immunocytochemistry
  • Expression of TRPV3 in rat DRG primary culture
    Expression of TRPV3 in rat DRG primary culture
    Cell surface detection of TRPV3 in living rat dorsal root ganglion (DRG) primary culture using Anti-TRPV3 (extracellular) Antibody (#ACC-033), (1:50-1:100), followed by goat anti-rabbit-AlexaFluor-555 secondary antibody (A). B. Nuclear staining with the cell-permeable dye Hoechst 33342. C. Merged image of panels A and B.
References
  1. Montell, C. et al. (2002) Mol. Cell. 9, 229.
  2. Clapham, D.E. (2003) Nature 426, 517.
  3. Moran, M.M. et al. (2004) Curr. Opin. Neurobiol. 14, 362.
  4. Clapham, D.E. et al. (2003) Pharmacol. Rev. 55, 591.
  5. Gunthorpe, M.J. et al. (2002) Trends. Pharmacol. Sci. 23, 183.
  6. Tominaga, M. and Caterina, M.J. et al. (2004) J. Neurobiol. 61, 3.
  7. Voets, T. et al. (2004) Nature 430, 748.
  8. Xiao, R. et al. (2008) J. Biol. Chem. 283, 6162.
Scientific background

TRP channels are a large family (about 28 genes) of plasma membrane, non-selective cationic channels that are either specifically or ubiquitously expressed in excitable and non-excitable cells.1 The TRP channels have six putative transmembrane domains (TM) with a pore domain between the fifth and the sixth TM, and all assemble as tetramers. Both the N- and the C-terminus of all TRPs are intracellular.3

According to IUPHAR, the TRP family is composed of three main subfamilies on the basis of sequence homology; TRPC, TRPM and TRPV (to date, three additional subfamilies are also considered to belong to the TRP family: the TRPA, TRPML, and TRPP).1-4 The TRPV subfamily consists of six members, TRPV1-6.5

Four members of the TRPV family have been described as a thermosensitive ion channels (TRPV1 to TRPV4). Each channel exhibits distinct thermal activation thresholds ranging from noxious cold (<17°C) to noxious heat (>52°C).6,7

TRPV3 is highly expressed in skin keratinocytes. It forms a nonselective cation channel that is activated by hot temperatures in the innocuous and noxious range. The channel is also expressed in oral and nasal cavities where it has been postulated to play a role in flavor sensation. TRPV3 is also implicated as a molecular target of some allergens and skin sensitizers.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 16/08/2020

Anti-TRPV3 (extracellular) Antibody (#ACC-033) is a highly specific antibody directed against an extracellular epitope of human TRPV3. The antibody can be used in western blot, immunohistochemical and immunocytochemical applications. As the antibody recognizes an extracellular epitope, it can be used as a tool to detect TRPV3 in live cells. The antibody has been designed to recognize TRPV3 from human, rat and mouse samples.

For research purposes only, not for human use
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