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Anti-TSH Receptor (TSHR) (extracellular)-ATTO Fluor-488 Antibody

Thyrotropin receptor, Thyroid-stimulating hormone receptor, TSH-R

Cat #: ATR-006-AG
Alternative Name Thyrotropin receptor, Thyroid-stimulating hormone receptor, TSH-R
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h, m, r
Immunogen
  • Peptide ETLQAFDSHYDYTVC, corresponding to amino acid residues 376-390 of rat TSHR (Accession P21463). Extracellular, N-terminus.
Accession (Uniprot) Number P21463
Gene ID 25360
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Mouse, human - identical.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG.
Label ATTO-488. Maximum absorption 501 nm; maximum fluorescence 523 nm. The fluorescence is excited most efficiently in the 480 - 515 nm range. This label is analogous to the dye fluorescein isothiocyanate (FITC) and can be used with filters used to detect FITC.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 50 µl double distilled water (DDW).
Antibody concentration after reconstitution 1 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 × g 5 min).
Standard quality control of each lot Western blot analysis (unlabeled antibody, #ATR-006), and immunohistochemical staining (labeled antibody).
Applications: if, ih
May also work in: ic*, lci*
Immunohistochemistry
  • Expression of TSH receptor in mouse brain
    Expression of TSH receptor in mouse brain
    Immunohistochemical staining of TSHR in mouse brain sections using Anti-TSH Receptor (TSHR) (extracellular)-ATTO Fluor-488 Antibody (#ATR-006-AG), (1:60). A. In the cerebellum, TSHR staining (green) appears in the molecular layer (MOL) and purkinje (P) layer. B. In the hippocampus, staining appears in CA3 pyramidal layer (P), (arrows). Nuclei are stained using DAPI (blue).
References
  1. Klein, J.R. (2014) Front. Immunol7, 155.
  2. Vassart, G. and Dumont, J.E. (1992) Endocr. Rev. 13, 596.
  3. de Lloyd A. et al. (2010) J. Endocrinol. 204, 13.
  4. Davies, T. et al. (2002) J. Clin. Invest. 110, 161.
  5. Prabhakar, B.S. et al. (2003) Endocr. Rev. 24, 802.
Scientific background

Thyroid stimulating hormone (TSH), a glycoprotein hormone composed of α and β chains, is produced by thyrotrope cells of the anterior pituitary1. The TSH receptor (TSHR) plays a prominent role in thyroid physiology and disease. The established biological function of TSHR in the thyroid gland is to regulate synthesis and secretion of thyroid hormones from follicular thyroid cells; it also plays an important role in controlling the growth and development of the thyroid gland2.

TSHR is a G-protein coupled receptor and shares the classic structure of the serpentine receptor family (seven membrane spanning segments, an extracellular N-terminus and an intracellular carboxy terminal)3.

TSHR is primarily expressed in the epithelial cells of the thyroid follicles, but TSHR mRNA and protein have been detected in a variety of cell types including brain, testes, kidney, heart, bone, thymus, lymphocytes, adipose tissue and fibroblasts4.

Chronic stimulation of the TSHR leads to over activation of the cAMP pathway that in turn causes thyroid hyperplasia and hyperthyroidism. This process occurs in Graves' disease (GD) in which thyroid stimulating antibodies (TSAB) bind the receptor and mimic the action of TSH5.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 24/01/2021

Anti-TSH Receptor (TSHR) (extracellular) Antibody (#ATR-006) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot analysis. The antibody recognizes an extracellular epitope and thus is ideal for detecting the receptor in living cells. It has been designed to recognize TSHR from human, rat, and mouse samples.

Anti-TSH Receptor (TSHR) (extracellular)-ATTO Fluor-488 Antibody (#ATR-006-AG) is directly labeled with an ATTO-488 fluorescent dye. ATTO dyes are characterized by strong absorption (high extinction coefficient), high fluorescence quantum yield, and high photo-stability. The ATTO-488 label is analogous to the fluorescein isothiocyanate dye (FITC) and can be used with filters typically used to detect FITC. Anti-TSH Receptor (TSHR) (extracellular)-ATTO Fluor-488 Antibody has been tested in immunohistochemistry applications and is especially suited for experiments requiring simultaneous labeling of different markers.

For research purposes only, not for human use

Applications

Specifications

Scientific Background

Citations

Citations
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