This product has recently been released.
Cat #: ATR-006-AG
This product is freeze dried. All water molecules have been removed.
This antibody is shipped with its antigen FREE of charge!
Reactivity: h, m, r
Peptide ETLQAFDSHYDYTVC, corresponding to amino acid residues 376-390 of rat TSH receptor (Accession P21463). Extracellular, N-terminus.
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Mouse, human - identical.
Purity Affinity purified on immobilized antigen.
Formulation Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Label ATTO-488. Maximum absorption 501 nm; maximum fluorescence 523 nm. The fluorescence is excited most efficiently in the 480 - 515 nm range. This label is analogous to the dye fluorescein isothiocyanate (FITC) and can be used with filters used to detect FITC.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 50 µl double distilled water (DDW).
Antibody concentration after reconstitution 1 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 × g 5 min).
Control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Control antigen reconstitution 100 µl double distilled water (DDW).
Control antigen storage after reconstitution -20°C.
Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard quality control of each lot Western blot analysis (unlabeled antibody, #ATR-006), and immunohistochemical staining (labeled antibody).
May also work in: ic, lci
Mouse brain sections (1:60).
Expression of TSH Receptor in mouse brain
Immunohistochemical staining of TSH receptor in mouse brain sections using Anti-TSH Receptor (extracellular)-ATTO-488 Antibody (#ATR-006-AG), (1:60). A. In the cerebellum, TSH receptor staining (green) appears in the molecular layer (MOL) and purkinje (P) layer. B. In the hippocampus, staining appears in CA3 pyramidal layer (P), (arrows). Nuclei are stained using DAPI (blue).
CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot
Species reactivity key:
H- Human, M- Mouse, R- Rat
Lyophilized PowderControl Antigen Included
Last update: 03/02/2019
For research purposes only, not for human use