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Home › Products › G-Protein Coupled Receptors › Glycoprotein Hormone Receptors › TSH Receptor › Antibodies

Certificate of Analysis

Anti-TSH Receptor (extracellular)-ATTO-488 Antibody
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Thyroid-stimulating hormone receptor, TSH-R, Thyrotropin receptor
  • New
    New

    This product has recently been released.

Cat #: ATR-006-AG
Alternative Name Thyroid-stimulating hormone receptor, TSH-R, Thyrotropin receptor
  • Lyophilized Powder
    Lyophilized Powder

    This product is freeze dried. All water molecules have been removed.

  • Antigen Incl.
    Antigen Incl.

    This antibody is shipped with its antigen FREE of charge!

    • Type: Polyclonal
    Source: Rabbit
    Reactivity: h, m, r
      • Peptide ETLQAFDSHYDYTVC, corresponding to amino acid residues 376-390 of rat TSH receptor (Accession P21463). Extracellular, N-terminus.
    Accession (Uniprot) Number P21463
    Gene ID 25360
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Mouse, human - identical.
    Purity Affinity purified on immobilized antigen.
    Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Label ATTO-488. Maximum absorption 501 nm; maximum fluorescence 523 nm. The fluorescence is excited most efficiently in the 480 - 515 nm range. This label is analogous to the dye fluorescein isothiocyanate (FITC) and can be used with filters used to detect FITC.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 50 µl double distilled water (DDW).
    Antibody concentration after reconstitution 1 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 × g 5 min).
    Control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
    Control antigen reconstitution 100 µl double distilled water (DDW).
    Control antigen storage after reconstitution -20°C.
    Preadsorption Control 1 µg peptide per 1 µg antibody.
    Standard quality control of each lot Western blot analysis (unlabeled antibody, #ATR-006), and immunohistochemical staining (labeled antibody).
    Applications: ih
    May also work in: ic, lci
      • Mouse brain sections (1:60).
        Expression of TSH Receptor in mouse brain
        Immunohistochemical staining of TSH receptor in mouse brain sections using Anti-TSH Receptor (extracellular)-ATTO-488 Antibody (#ATR-006-AG), (1:60). A. In the cerebellum, TSH receptor staining (green) appears in the molecular layer (MOL) and purkinje (P) layer. B. In the hippocampus, staining appears in CA3 pyramidal layer (P), (arrows). Nuclei are stained using DAPI (blue).

      • Thyroid stimulating hormone (TSH), a glycoprotein hormone composed of α and β chains, is produced by thyrotrope cells of the anterior pituitary1. The TSH receptor (TSHR) plays a prominent role in thyroid physiology and disease. The established biological function of TSHR in the thyroid gland is to regulate synthesis and secretion of thyroid hormones from follicular thyroid cells; it also plays an important role in controlling the growth and development of the thyroid gland2.

        TSHR is a G-protein coupled receptor and shares the classic structure of the serpentine receptor family (seven membrane spanning segments, an extracellular N-terminus and an intracellular carboxy terminal)3.

        TSHR is primarily expressed in the epithelial cells of the thyroid follicles, but TSHR mRNA and protein have been detected in a variety of cell types including brain, testes, kidney, heart, bone, thymus, lymphocytes, adipose tissue and fibroblasts4.

        Chronic stimulation of the TSHR leads to over activation of the cAMP pathway that in turn causes thyroid hyperplasia and hyperthyroidism. This process occurs in Graves' disease (GD) in which thyroid stimulating antibodies (TSAB) bind the receptor and mimic the action of TSH5.

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Lyophilized PowderControl Antigen Included
    Back to product page MSDS
    Last update: 04/04/2019
    For research purposes only, not for human use
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