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Anti-TSH Receptor (TSHR) (extracellular) Antibody

Thyrotropin receptor, Thyroid-stimulating hormone receptor, TSH-R

Cat #: ATR-006
Alternative Name Thyrotropin receptor, Thyroid-stimulating hormone receptor, TSH-R
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h, m, r
Immunogen
  • Peptide ETLQAFDSHYDYTVC, corresponding to amino acid residues 376-390 of rat TSHR (Accession P21463). Extracellular, N-terminus.
Accession (Uniprot) Number P21463
Gene ID 25360
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Mouse, human - identical.
RRID AB_2341080.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.85 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: ifc, ih, lci, wb
May also work in: ic*, ip*
Western blot
  • Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain lysates:
    Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain lysates:
    1,2. Anti-TSH Receptor (TSHR) (extracellular) Antibody (#ATR-006), (1:200).
    3,4. Anti-TSH Receptor (TSHR) (extracellular) Antibody, preincubated with TSH Receptor/TSHR (extracellular) Blocking Peptide (#BLP-TR006).
Immunohistochemistry
  • Expression of TSH Receptor in mouse hippocampus.
    Expression of TSH Receptor in mouse hippocampus.
    Immunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-TSH Receptor (TSHR) (extracellular) Antibody (#ATR-006), (1:200), followed by goat anti-rabbit-AlexaFluor-488. A. Staining in the hippocampal CA3 region, showed immunoreactivity (green) in the pyramidal layer (P, arrows) and in mossy fiber terminal region (MF). B. Pre-incubation of the antibody with TSH Receptor (TSHR) (extracellular) Blocking Peptide (BLP-TR006), suppressed staining. Cell nuclei are stained with DAPI (blue).
Indirect flow cytometry
  • Cell surface detection of TSH Receptor by indirect flow cytometry in live intact human THP-1 monocytic leukemia cells: 
    Cell surface detection of TSH Receptor by indirect flow cytometry in live intact human THP-1 monocytic leukemia cells: 
    ___ Cells.
    ___ Cells + goat-anti-rabbit-FITC.
    ___ Cells + Anti-TSH Receptor (TSHR) (extracellular) Antibody(#ATR-006), (2.5μg) + goat-anti-rabbit-FITC.
References
  1. Klein, J.R. (2014) Front. Immunol. 7, 155.
  2. Vassart, G. and Dumont, J.E. (1992) Endocr. Rev. 13, 596.
  3. de Lloyd A. et al. (2010) J. Endocrinol. 204, 13.
  4. Davies, T. et al. (2002) J. Clin. Invest. 110, 161.
  5. Prabhakar, B.S. et al. (2003) Endocr. Rev. 24, 802.
Scientific background

Thyroid stimulating hormone (TSH), a glycoprotein hormone composed of α and β chains, is produced by thyrotrope cells of the anterior pituitary1. The TSH receptor (TSHR) plays a prominent role in thyroid physiology and disease. The established biological function of TSHR in the thyroid gland is to regulate synthesis and secretion of thyroid hormones from follicular thyroid cells; it also plays an important role in controlling the growth and development of the thyroid gland2.

TSHR is a G-protein coupled receptor and shares the classic structure of the serpentine receptor family (seven membrane spanning segments, an extracellular N-terminus and an intracellular carboxy terminal)3.

TSHR is primarily expressed in the epithelial cells of the thyroid follicles, but TSHR mRNA and protein have been detected in a variety of cell types including brain, testes, kidney, heart, bone, thymus, lymphocytes, adipose tissue and fibroblasts4.

Chronic stimulation of the TSHR leads to over activation of the cAMP pathway that in turn causes thyroid hyperplasia and hyperthyroidism. This process occurs in Graves' disease (GD) in which thyroid stimulating antibodies (TSAB) bind the receptor and mimic the action of TSH5.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 13/04/2021

Anti-TSH Receptor (TSHR) (extracellular) Antibody (#ATR-006) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot analysis. The antibody recognizes an extracellular epitope and thus is ideal for detecting the receptor in living cells. It has been designed to recognize TSHR from human, rat, and mouse samples.

For research purposes only, not for human use

Applications

Specifications

Scientific Background

Citations

Citations
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