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Anti-Urotensin II Receptor Antibody

Urotensin 2 receptor, UR-II-R, UR2R, UTS2R, SENR, GPR14
Cat #: AER-003
Alternative Name Urotensin 2 receptor, UR-II-R, UR2R, UTS2R, SENR, GPR14
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: r
May also work in: h*
Immunogen
  • Peptide (C)RPLDTVQRSKGYRK, corresponding to amino acid residues 154-167 of rat URIIR (Accession P49684). 2nd intracellular loop.
Accession (Uniprot) Number P49684
Gene ID 57305
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Bovine - identical; human, canine - 13/14 amino acid residues identical.
RRID AB_2039849.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 1 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: ih, wb
May also work in: ic*, ifc*, ip*
Western blot
  • Western blot analysis of rat kidney membranes:
    Western blot analysis of rat kidney membranes:
    1. Anti-Urotensin II Receptor Antibody (#AER-003), (1:200).   
    2. Anti-Urotensin II Receptor Antibody, preincubated with Urotensin II Receptor Blocking Peptide (#BLP-ER003).
Immunohistochemistry
  • Rat paraffin kidney section.
References
  1. Davenport, A.P. et al. (2002) Pharmacol. Rev. 54, 219.
  2. Boivin, B. et al. (2003) J. Biol. Chem. 278, 29153.
  3. Maguire, J.J. et al. (2000) Br. J. Pharmacol. 131, 441.
  4. Matsushita, M. et al. (2001) J. Hypertens. 19, 2185.
  5. Maguire, J.J. and Davenport, A.P. (2002) Br. J. Pharmacol. 137, 57.
  6. Ziltener, P. et al. (2002) J. Recept. Signal Transduct. Res. 22, 155.
  7. Takahashi, K. et al. (2000) Clin. Sci. 103, 35S.
  8. Takahashi, K. et al. (2001) Peptides 22, 1175.
  9. Takahashi, K. et al. (2003) Peptides 24, 301.
Scientific background Urotensin II is a powerful vasoconstrictive substance. The human urotensin II receptor (UR-II-R) was originally isolated as an orphan receptor (GPR14) from neural and sensory tissues.1,2 Urotensin II is expressed in various human tissues such as the CNS, skeletal muscle, smooth muscle, pancreas, and heart.3-5 Binding of urotensin II to the receptor leads to activation of phospholipase C. The resulting increase in intracellular Ca2+ may cause the contraction of vascular smooth muscle. ERK1/2 phophorylation may also be involved in mediating the biological activities of urotensin II.Recently, evidence has accumulated indicating that urotensin II is also expressed in various kinds of tumor cells such as adrenal tumors and neuroblastomas. In those cells, urotensin II was reported to stimulate cell growth.7-9

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 16/08/2020

Alomone Labs is pleased to offer a highly specific antibody directed against the 2nd cytoplasmic loop of the rat UTS2R. Anti-Urontenisn II Receptor Antibody (#AER-003) can be used in western blot and immunohistochemical applications, and recognizes UTS2R in rat samples.

For research purposes only, not for human use

Applications

Specifications

Scientific Background

Citations

Citations
More product citations
  1. Soni, H. and Adebiyi, A. (2013) J. Endocrinol. 217, 317.
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