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Guinea pig Anti-Vesicular GABA Transporter (VGAT) Antibody

Vesicular inhibitory amino acid transporter, VIAAT, GABA and glycine transporter, rGVAT, SLC32A1

Cat #: AGP-129
Alternative Name Vesicular inhibitory amino acid transporter, VIAAT, GABA and glycine transporter, rGVAT, SLC32A1
Lyophilized Powder yes
Type: Polyclonal
Host: Guinea pig
Reactivity: h, m, r
Immunogen
  • Peptide (C)GEFGGHDKPKITAWE, corresponding to amino acid residues 106-120 of rat vesicular GABA transporter (Accession O35458). Cytoplasmic, N-terminus.
Accession (Uniprot) Number O35458
Gene ID 83612
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Mouse, human - identical.
RRID AB_2756624.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Guinea pig total IgG.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.8 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: wb
May also work in: ic*, ifc*, ih*, ip*
Western blot
  • Western blot analysis of rat brain membranes (lanes 1 and 5), mouse brain membranes (lanes 2 and 6), human U-87 MG glyoblastoma lysates (lanes 3 and 7) and human SH-SY5Y brain neuroblastoma lysates (lanes 4 and 8):
    Western blot analysis of rat brain membranes (lanes 1 and 5), mouse brain membranes (lanes 2 and 6), human U-87 MG glyoblastoma lysates (lanes 3 and 7) and human SH-SY5Y brain neuroblastoma lysates (lanes 4 and 8):
    1-4. Guinea pig Anti-Vesicular GABA Transporter (VGAT) Antibody (#AGP-129), (1:200).
    5-8. Guinea pig Anti-Vesicular GABA Transporter (VGAT) Antibody, preincubated with Vesicular GABA Transporter/VGAT Blocking Peptide (#BLP-GT005).
References
  1. Gasnier, B. (2004) Pflugers. Arch. 447, 756.
  2. McIntire, S. et al. (1997) Nature. 389, 870.
  3. Bedet, C. et al. (2000) J. Neurochem. 75, 1654.
  4. Chaudry, F. et al. (1998) J. Neurosci. 18, 9733.
  5. Dumoulin, A. et al. (1999) J. Cell. Sci. 112, 811.
  6. Takamori, S. et al. (2000) J. Neurosci. 20, 4904.
  7. Mayerhofer, A. et al. (2001) FASEB J. 15, 1089.
  8. Chessler, S. et al. (2002) Diabetes. 51, 1763.
Scientific background

The superfamily of amino acid transporters includes SLC32, SLC36 and SLC38 families. SLC32 has only one member, the vesicular inhibitory amino acid transporter (VIAAT) or vesicular GABA transporter (VGAT)1.

VGAT is responsible for the uptake of GABA and glycine in exchange of protons, and their release from nerve terminals1.

The transporter is organized into ten membrane spanning domains, a cytosolic N-terminus and a luminal C-terminal tail2. In many regions in the brain, where it is expressed, it is found constitutively phosphorylated, although the biological significance is currently unknown3.

VGAT is expressed on synaptic vesicles of GABAergic and glycynergic neurons4-6. The transporter is also detected in the pituitary gland and in the pancreas7,8.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 02/03/2021

Guinea pig Anti-Vesicular GABA Transporter (VGAT) Antibody (#AGP-129), raised in guinea pigs, is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot analysis. It has been designed to recognize VGAT from mouse, rat, and human samples. The antigen used to immunize guinea pigs is the same as Anti-Vesicular GABA Transporter (VGAT) Antibody (#AGT-005) raised in rabbit. Our line of guinea pig antibodies enables more flexibility with our products such as multiplex staining studies, immunoprecipitation, etc.

For research purposes only, not for human use

Applications

Specifications

Scientific Background

Citations

Citations
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