- Peptide (C)GEFGGHDKPKITAWE, corresponding to amino acid residues 106-120 of rat vesicular GABA transporter (Accession O35458). Cytoplasmic, N-terminus.
- Rat and mouse brain membranes, human U-87 MG glioblastoma, and SH-SY5Y brain neuroblastoma lysates (1:200-1:1000).
- Western blot analysis of rat brain membranes (lanes 1 and 5), mouse brain membranes (lanes 2 and 6), human U-87 MG glyoblastoma lysates (lanes 3 and 7) and human SH-SY5Y brain neuroblastoma lysates (lanes 4 and 8):1-4. Guinea pig Anti-Vesicular GABA Transporter (VGAT) Antibody (#AGP-129), (1:200).
5-8. Guinea pig Anti-Vesicular GABA Transporter (VGAT) Antibody, preincubated with Vesicular GABA Transporter/VGAT Blocking Peptide (#BLP-GT005).
The superfamily of amino acid transporters includes SLC32, SLC36 and SLC38 families. SLC32 has only one member, the vesicular inhibitory amino acid transporter (VIAAT) or vesicular GABA transporter (VGAT)1.
VGAT is responsible for the uptake of GABA and glycine in exchange of protons, and their release from nerve terminals1.
The transporter is organized into ten membrane spanning domains, a cytosolic N-terminus and a luminal C-terminal tail2. In many regions in the brain, where it is expressed, it is found constitutively phosphorylated, although the biological significance is currently unknown3.
VGAT is expressed on synaptic vesicles of GABAergic and glycynergic neurons4-6. The transporter is also detected in the pituitary gland and in the pancreas7,8.