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Home › Products › Transporters, Exchangers and Pumps › Glutamate Transporters › Antibodies

Certificate of Analysis

Anti-VGLUT2 Antibody

Vesicular glutamate transporter 2, Differentiation-associated Na+-dependent inorganic phosphate cotransporter, DNPI, SLC17A6

Cat #: AGC-036
Alternative Name Vesicular glutamate transporter 2, Differentiation-associated Na+-dependent inorganic phosphate cotransporter, DNPI, SLC17A6
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h, m, r
Immunogen
  • Peptide (C)EDGKPLEVPEKK, corresponding to amino acid residues 45-56 of rat VGLUT2 (Accession Q9JI12). Cytoplasmic, N-terminus.
Accession (Uniprot) Number Q9JI12
Gene ID 84487
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Mouse - identical; human - 11/12 amino acid residues identical.
RRID AB_2340950.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 μl, 50 μl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.8 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: if, ih, wb
May also work in: ic*, ifc*, ip*
Western blot
  • Rat and mouse brain lysates. Human SH-SY5Y cell line (1:400-1:1500).
  • Western blot analysis of rat brain membranes (lanes 1 and 4), mouse brain membranes (lanes 2 and 5) and human SH-SY5Y neuroblastoma cell lysate (lanes 3 and 6):
    Western blot analysis of rat brain membranes (lanes 1 and 4), mouse brain membranes (lanes 2 and 5) and human SH-SY5Y neuroblastoma cell lysate (lanes 3 and 6):
    1-3. Anti-VGLUT2 Antibody (#AGC-036), (1:400).
    4-6. Anti-VGLUT2 Antibody, preincubated with VGLUT2 Blocking Peptide (#BLP-GC036).
Immunohistochemistry
  • Rat brain frozen sections (1:400). Rat spinal cord (1:600).
Scientific background

Central nervous system neurons have traditionally been thought to express exclusively membrane transporters and/or vesicular transporters for their transmitter. Three vesicular glutamate transporters (VGLUTs) have been cloned: BNPI/VGLUT1 (a brain-specific sodium dependent inorganic phosphate (Pi) transporter), and its homologs DNPI/VGLUT2 (differentiation-associated sodium-dependent Pi transporter) and VGLUT31. These transporters mediate glutamate uptake inside presynaptic vesicles and are anatomical and functional markers of glutamatergic excitatory transmission2.

BNPI/DNPI encodes a membrane protein with 6-8 putative transmembrane domains which exhibits weak similarities to a kidney Na+-dependent inorganic phosphate co-transporter3. The transporters use a membrane potential gradient set by the vesicular H+-ATPase for glutamate uptake4. VGLUT1-3 are very similar in structure and function, but are used by different neuronal populations. VGLUT1 and VGLUT2 are expressed by the cortical and subcortical neurons respectively. VGLUT3 is expressed by non-glutamatergic neurons5. VGLUT2 is expressed in the thalamus, brainstem, and deep cerebellar nuclei2. A Recent study has shown that  targeted deletion of VGLUT2 in mice causes perinatal lethality and a 95% reduction in evoked glutamatergic responses in thalamic neurons, although hippocampal synapses function normally. Behavioral analysis of heterozygous VGLUT2 mice showed discrete behavioral phenotypes that suggest a deficit in thalamic processing6.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Lyophilized Powder
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Image & Title:

Anti-VGLUT2 AntibodyMultiplex staining of VGLUT2 and P2X7 Receptor in rat spinal cordImmunohistochemical staining of perfusion-fixed frozen rat spinal cord sections using Anti-VGLUT2 Antibody (#AGC-036), (1:600) and Anti-P2X7 Receptor-ATTO Fluor-550 Antibody (#APR-004-AO), (1:100). A. VGLUT2 labeling followed by goat-anti-rabbit-Alexa-488 (green). B. The same section labeled for P2X7 receptor (orange). C. Merge of A and B demonstrates partial co-localization of Vesicular glutamate transporter 2 and P2X7 receptor in the ventral horn (arrow). Cell nuclei were stained with DAPI (blue).

Last update: 08/06/2022

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For research purposes only, not for human use

Specifications

Citations

Citations

Applications

Scientific Background

Shipping and Ordering information
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    Control Product

    • A Blocking peptide for Anti-VGLUT2 Antibody (#AGC-036) and Guinea Pig Anti-VGLUT2 Antibody (#AGC-036-GP). Intended to be used as a negative control.

      VGLUT2 Blocking Peptide

    General Protocols

    • Blocking Peptides – Controls for better results
    • Blocking Peptide Protocol for Western Blot (WB)
    • Sample Preparation for Cell Lines
    • Immunohistochemistry (IHC) Protocols for Frozen Sections: Indirect Methods
    • Sample Preparation Protocols for Tissues
    • Western Blot (WB) Protocol

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