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Anti-VPAC2 (VIPR2) (extracellular) Antibody

VIP and PACAP receptor 2, Vasoactive intestinal polypeptide receptor 2, Helodermin-preferring VIP receptor, Pituitary adenylate cyclase-activating polypeptide type III receptor, PACAP type III receptor, PACAP-R3
Cat #: AVR-002
Alternative Name VIP and PACAP receptor 2, Vasoactive intestinal polypeptide receptor 2, Helodermin-preferring VIP receptor, Pituitary adenylate cyclase-activating polypeptide type III receptor, PACAP type III receptor, PACAP-R3
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h, m, r
Immunogen
  • Peptide CRFHLEIQEEETK, corresponding to amino acid residues 25-37 of human VPAC2 (Accession P41587). Extracellular, N-terminus.
Accession (Uniprot) Number P41587
Gene ID 7434
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Mouse, rat – identical.
RRID AB_2341082.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.8 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: ic, if, ifc, lci, wb
May also work in: ih*, ip*
Western blot
  • Western blot analysis of rat brain lysate (lanes 1 and 4), mouse brain lysate (lanes 2 and 5), and human Malme-3M melanoma cell lysate (lanes 3 and 6):
    Western blot analysis of rat brain lysate (lanes 1 and 4), mouse brain lysate (lanes 2 and 5), and human Malme-3M melanoma cell lysate (lanes 3 and 6):
    1-3. Anti-VPAC2 (VIPR2) (extracellular) Antibody (#AVR-002), (1:600).
    4-6. Anti-VPAC2 (VIPR2) (extracellular) Antibody, preincubated with VPAC2/VIPR2 (extracellular) Blocking Peptide (#BLP-VR002).
Indirect flow cytometry
  • Cell surface detection of VPAC2 in live intact human Jurkat cell line:
    Cell surface detection of VPAC2 in live intact human Jurkat cell line:
    ___ Cells + goat-anti-rabbit-DyLight-488.
    ___ Cells + Anti-VPAC2 (VIPR2) (extracellular) Antibody (#AVR-002), (1:15) + goat-anti-rabbit-DyLight-488.
Live cell imaging / Immunocytochemistry
  • Expression of VPAC2 in rat PC12 cells
    Expression of VPAC2 in rat PC12 cells
    Cell surface detection of VPAC2 in live intact rat pheochromocytoma (PC12) cells. A. Extracellular staining of cells with Anti-VPAC2 (VIPR2) (extracellular) Antibody (#AVR-002), (1:50), followed by goat anti-rabbit-AlexaFluor-594 secondary antibody (red). B. Live view of the cells. C. Merge of A and B.
References
  1. Laburthe, M. et al. (2007) Peptides 28, 1631.
  2. Reubi, J.C. (2006) Ann. N.Y. Acad. Sci. 921, 1.
  3. Soetedjo, L. et al. (2013) Biol. Open. 2, 686.
  4. Aton, S.J. et al. (2005) Nat. Neurosci. 8, 476.
  5. Beri, S. et al. (2012) Eur. J. Hum. Genet. 21, 757.
Scientific background

Vasoactive intestinal peptide (VIP) and pituitary adenylate cyclase-activating peptide (PACAP) are prominent neuropeptides that are structurally related. Two receptors have similarly high affinity for VIP and PACAP and have therefore been termed VIP and PACAP receptor 1 (VPAC1) and VIP and PACAP receptor 2 (VPAC2). VPAC2 (also known as vasoactive intestinal peptide receptor 2) belongs to the Class II family of G-protein coupled receptors1. A distinct feature of class II GPCRs is the presence of large N-terminal extracellular domains which contain 10 highly conserved amino acids including six cysteines, putative N terminal leader sequences and several potential N-glycosylation sites1.

VPAC2 receptor is expressed in the uterus, prostate, smooth muscle of the gastrointestinal tract, seminal vesicles and skin, blood vessels and thymus2. VPAC2 is also expressed in various brain regions including the thalamus and the suprachiasmatic nuclei (SCN)3.

VPAC2 plays important roles in the control of mammalian circadian rhythms in the SCN. Mice lacking VPAC2 show altered circadian rhythms in locomotor behavior, neuronal firing and clock gene expression4. Recent studies have also shown that duplication of the VPAC2 gene, and the resulting higher than normal VPAC2 signaling in patients confers a significant risk to schizophrenia5.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 23/08/2020

Alomone Labs is pleased to offer a highly specific antibody directed against an extracellular epitope of human VIP and PACAP receptor 2. Anti-VPAC2 (VIPR2) (extracellular) Antibody (#AVR-002) can be used in western blot analysis, immunocytochemistry and indirect flow cytometry applications. It has been designed to recognize VPAC2 from human, rat and mouse samples.

For research purposes only, not for human use

Applications

Specifications

Scientific Background

Citations

Citations
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