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Anti-ZIP8 (SLC39A8) (extracellular) Antibody

Zinc transporter ZIP8, Zrt- and Irt-like protein 8, Solute carrier family 39 member 8, BIGM103

Cat #: AZT-008
Alternative Name Zinc transporter ZIP8, Zrt- and Irt-like protein 8, Solute carrier family 39 member 8, BIGM103
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h, m, r
Immunogen
  • Peptide (C)KTYGQNDHTHFRND, corresponding to amino acid residues 221-234 of rat ZIP8 (Accession Q5FVQ0). 2nd extracellular loop.
Accession (Uniprot) Number Q5FVQ0
Gene ID 295455
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Mouse – identical; human – 12/14 amino acid residues identical.
RRID AB_2756810.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.8 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: if, ifc, ih, wb
May also work in: ic*, ip*, lci*
Western blot
  • Western blot analysis of mouse kidney (lanes 1 and 3) and mouse brain (lanes 2 and 4) lysates:
    Western blot analysis of mouse kidney (lanes 1 and 3) and mouse brain (lanes 2 and 4) lysates:
    1,2. Anti-ZIP8 (SLC39A8) (extracellular) Antibody (#AZT-008), (1:200).
    3,4. Anti-ZIP8 (SLC39A8) (extracellular) Antibody, preincubated with ZIP8/SLC39A8 (extracellular) Blocking Peptide (#BLP-ZT008).
  • Western blot analysis of rat brain lysate:
    Western blot analysis of rat brain lysate:
    1. Anti-ZIP8 (SLC39A8) (extracellular) Antibody (#AZT-008), (1:200).
    2. Anti-ZIP8 (SLC39A8) (extracellular) Antibody, preincubated with ZIP8/SLC39A8 (extracellular) Blocking Peptide (#BLP-ZT008).
  • Western blot analysis of human Jurkat T-cell leukemia cell line (lanes 1 and 3) and human K562 chronic myelogenous leukemia cell line (lanes 2 and 4) lysates:
    Western blot analysis of human Jurkat T-cell leukemia cell line (lanes 1 and 3) and human K562 chronic myelogenous leukemia cell line (lanes 2 and 4) lysates:
    1,2. Anti-ZIP8 (SLC39A8) (extracellular) Antibody (#AZT-008), (1:200).
    3,4. Anti-ZIP8 (SLC39A8) (extracellular) Antibody, preincubated with ZIP8/SLC39A8 (extracellular) Blocking Peptide (#BLP-ZT008).
Immunohistochemistry
  • Expression of Zinc transporter ZIP8 in rat hippocampus
    Expression of Zinc transporter ZIP8 in rat hippocampus
    Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-ZIP8 (SLC39A8) (extracellular) Antibody (#AZT-008), (1:200), followed by goat-anti-rabbit-AlexaFluor-488. ZIP8 staining (green) appears in interneurons (arrows) adjacent to the granular layer (GL). Cell nuclei are stained with DAPI (blue).
Indirect flow cytometry
  • Cell surface detection of Zinc transporter ZIP8 in live intact human THP-1 acute monocytic leukemia cells:
    Cell surface detection of Zinc transporter ZIP8 in live intact human THP-1 acute monocytic leukemia cells:
    ___ Cells.
    ___ Cells + goat-anti-rabbit-FITC.
    ___ Cells + Anti-ZIP8 (SLC39A8) (extracellular) Antibody (#AZT-008), (2.5 µg) + goat-anti-rabbit-FITC.
References
  1. Jeong, J. and Eide, D.J. (2013) Mol. Aspects Med. 34, 612.
  2. Aydemir, T.B. et al. (2009) J. Leukoc. Biol. 86, 337.
Scientific background

Zinc uptake transporter, ZIP8, belongs to the SLC39 transporter family. ZIP transporters are responsible for zinc transport into the cytoplasm across cellular membranes, either influx from the extracellular space or efflux from intracellular organelles. Regulation and maintenance of proper concentration and distribution of cellular zinc nutrient by ZIP transporters are essential to the function, metabolism, growth, proliferation and survival of cells1,2.

ZIP structure contains eight predicted transmembrane (TM) domains while the N- and C-termini are extracellular. A long loop region is present between TM3 and TM4 domains, harboring a histidine-rich region. TM4 and TM5 domains are amphipathic and are thought to form a cavity through which metals are transported1.

ZIP8 protein is encoded by the SLC39A8 gene and is highly expressed in T cells. ZIP8 also plays an important role at the onset of inflammation2.

Zinc deficiency can cause growth retardation, cognitive impairment, metabolic disorders, infertility, dysfunction of the immune system, abnormalities in T-cells, natural killer cells and monocytes, and reduction in antibody formation1,2. Studies exploring knockdown of ZIP8 protein show a reduction of cellular zinc content, impaired mitochondrial function in response to TNF-α and increased cell death2.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 24/01/2021

Anti-ZIP8 (SLC39A8) (extracellular) Antibody (#AZT-008) is a highly specific antibody directed against an epitope of the rat Zinc transporter ZIP8. The antibody can be used in western blot, immunohistochemistry, and indirect live cell flow cytometry. It has been designed to recognize ZIP8 from human, rat, and mouse samples.

For research purposes only, not for human use

Applications

Specifications

Scientific Background

Citations

Citations
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