Overview
- Peptide (C)ENLEKKPRRTKAE, corresponding to amino acid residues 473-485 of rat ZnT1 (Accession Q62720). Intracellular, C-terminus.
Western blot analysis of mouse lung lysate (lanes 1 and 4), rat liver lysate (lanes 2 and 5) and rat brain membranes (lanes 3 and 6):1-3. Anti-ZnT1 (SLC30A1) Antibody (#AZT-011), (1:400).
4-6. Anti-ZnT1 (SLC30A1) Antibody, preincubated with ZnT1/SLC30A1 Blocking Peptide (#BLP-ZT011).
Expression of Zinc transporter 1 in rat olfactory bulbImmunohistochemical staining of perfusion-fixed frozen rat brain sections using Anti-ZnT1 (SLC30A1) Antibody (#AZT-011), (1:300), followed by goat anti-rabbit-AlexaFluor-488 antibody. ZnT1 staining (green) appears in glomeruli (G) and is particularly intense in the outer border (arrows). Cell nuclei are labeled with DAPI (blue).
- Hashimoto, A. and Kambe, T. (2015) J. Nutr. Sci. Vitaminol. 61, S116.
- Sindreu, C. et al. (2014) Mol. Brain. 7, 16.
- Langmade, S.J. et al. (2000) J. Biol. Chem. 275, 34803.
- McMahon, R.J. and Cousins, R.J. (1998) Proc. Natl. Acad. Sci. U.S.A. 95, 4841.
ZnT1 is a member of the SLC30 family of zinc transporters which promotes the influx of zinc ions into synaptic vesicles of glutamatergic neurons from the cytoplasm, intracellular organelles, and from the extracellular milieu.
Zinc plays an essential role in many physiological and pathological conditions such as synaptic plasticity modulation, memory formation and nociception by regulating transmitter receptors and signal transduction pathways. Therefore zinc homeostasis is tightly regulated at the cellular and the organismal levels1,2.
ZnT1 in mammals is localized primarily on the plasma membrane. Its role is to mediate zinc export to reduce intracellular zinc levels1. ZnT1 structure contains a PDZ I motif at the C-terminus and six transmembrane-spanning domains. There is also a (His-Gly)8 in the large intracellular loop between membrane-spanning regions 4 and 52,4.
ZnT1 expression is ubiquitous and is detected in the intestine, kidney and liver. In the intestine the expression is regional, being much greater in the duodenum and jejunum and in villus versus crypt cells.
The mechanism of ZnT1 regulation is under control of the zinc-responsive transcription factor MTF-11. Research shows that homozygous knockout of the ZnT1 gene is lethal to the foetus3.
Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of the rat Zinc transporter 1. Anti-ZnT1 (SLC30A1) Antibody (#AZT-011) can be used in western blot and immunohistochemistry applications. It has been designed to recognize ZnT1 from mouse, rat, and human samples.
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