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Anti-ZnT1 (SLC30A1) Antibody

Zinc transporter 1, ZRC 1

Cat #: AZT-011
Alternative Name Zinc transporter 1, ZRC 1
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: m, r
May also work in: h*
Immunogen
  • Peptide (C)ENLEKKPRRTKAE, corresponding to amino acid residues 473-485 of rat ZnT1 (Accession Q62720). Intracellular, C-terminus.
Accession (Uniprot) Number Q62720
Gene ID 58976
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Mouse, human  – 12/13 amino acid residues identical.
RRID AB_2715599.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.8 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: if, ih, wb
May also work in: ic*, ifc*, ip*
Western blot
  • Western blot analysis of mouse lung lysate (lanes 1 and 4), rat liver lysate (lanes 2 and 5) and rat brain membranes (lanes 3 and 6):
    Western blot analysis of mouse lung lysate (lanes 1 and 4), rat liver lysate (lanes 2 and 5) and rat brain membranes (lanes 3 and 6):
    1-3. Anti-ZnT1 (SLC30A1) Antibody (#AZT-011), (1:400).
    4-6. Anti-ZnT1 (SLC30A1) Antibody, preincubated with ZnT1/SLC30A1 Blocking Peptide (#BLP-ZT011).
Immunohistochemistry
  • Expression of Zinc transporter 1 in rat olfactory bulb
    Expression of Zinc transporter 1 in rat olfactory bulb
    Immunohistochemical staining of perfusion-fixed frozen rat brain sections using Anti-ZnT1 (SLC30A1) Antibody (#AZT-011), (1:300), followed by goat anti-rabbit-AlexaFluor-488 antibody. ZnT1 staining (green) appears in glomeruli (G) and is particularly intense in the outer border (arrows). Cell nuclei are labeled with DAPI (blue).
References
  1. Hashimoto, A. and Kambe, T. (2015) J. Nutr. Sci. Vitaminol. 61, S116.
  2. Sindreu, C. et al. (2014) Mol. Brain. 7, 16.
  3. Langmade, S.J. et al. (2000) J. Biol. Chem. 275, 34803.
  4. McMahon, R.J. and Cousins, R.J. (1998) Proc. Natl. Acad. Sci. U.S.A. 95, 4841.
Scientific background

ZnT1 is a member of the SLC30 family of zinc transporters which promotes the influx of zinc ions into synaptic vesicles of glutamatergic neurons from the cytoplasm, intracellular organelles, and from the extracellular milieu.

Zinc plays an essential role in many physiological and pathological conditions such as synaptic plasticity modulation, memory formation and nociception by regulating transmitter receptors and signal transduction pathways. Therefore zinc homeostasis is tightly regulated at the cellular and the organismal levels1,2.

ZnT1 in mammals is localized primarily on the plasma membrane. Its role is to mediate zinc export to reduce intracellular zinc levels1. ZnT1 structure contains a PDZ I motif at the C-terminus and six transmembrane-spanning domains. There is also a (His-Gly)8 in the large intracellular loop between membrane-spanning regions 4 and 52,4.

ZnT1 expression is ubiquitous and is detected in the intestine, kidney and liver. In the intestine the expression is regional, being much greater in the duodenum and jejunum and in villus versus crypt cells.

The mechanism of ZnT1 regulation is under control of the zinc-responsive transcription factor MTF-11. Research shows that homozygous knockout of the ZnT1 gene is lethal to the foetus3.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 02/03/2021

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of the rat Zinc transporter 1. Anti-ZnT1 (SLC30A1) Antibody (#AZT-011) can be used in western blot and immunohistochemistry applications. It has been designed to recognize ZnT1 from mouse, rat, and human samples.

For research purposes only, not for human use
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