Anti-ZIP6/SLC39A6 (extracellular) Antibody

Zinc transporter ZIP6, Zrt- and Irt-like protein 6, Estrogen-regulated protein LIV-1, LIV1, Solute carrier family 39 member 6
    Cat #: AZT-006
    Alternative Name Zinc transporter ZIP6, Zrt- and Irt-like protein 6, Estrogen-regulated protein LIV-1, LIV1, Solute carrier family 39 member 6
  • Lyophilized Powder
  • Antigen Incl.
  • Type: Polyclonal
    Host: Rabbit
    Reactivity: h, m, r
    Immunogen
    • Peptide (C)ESVSEPRKSFMYSR, corresponding to amino acid residues 251-264 of mouse ZIP6 (Accession Q8C145). Extracellular, N-terminus.
    • Anti-ZIP6/SLC39A6 (extracellular) Antibody
    Accession (Uniprot) Number Q8C145
    Gene ID 106957
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Rat, human – 13/14 amino acid residues identical.
    Purity Affinity purified on immobilized antigen.
    Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Isotype Rabbit IgG.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.8 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Negative control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
    Negative control antigen reconstitution 100 µl double distilled water (DDW).
    Negative control antigen storage after reconstitution -20°C.
    Preadsorption Control 1 µg peptide per 1 µg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: ifc, wb
    May also work in: ic*, ih*, ip*, lci*
    Western blot
    • Anti-ZIP6/SLC39A6 (extracellular) Antibody
      Western blot analysis of mouse (lanes 1 and 3) and rat (lanes 2 and 4) brain lysates:
      1,2. Anti-ZIP6/SLC39A6 (extracellular) Antibody (#AZT-006), (1:200).
      3,4. Anti-ZIP6/SLC39A6 (extracellular) Antibody, preincubated with the negative control antigen.
    • Anti-ZIP6/SLC39A6 (extracellular) Antibody
      Western blot analysis of human SH-SY5Y (lanes 1 and 3) and human K562 chronic myelogenous leukemia (lanes 2 and 4) cell line lysates:
      1,2. Anti-ZIP6/SLC39A6 (extracellular) Antibody (#AZT-006), (1:400).
      3,4. Anti-ZIP6/SLC39A6 (extracellular) Antibody, preincubated with the negative control antigen.
    Immunohistochemistry
    • Anti-ZIP6/SLC39A6 (extracellular) Antibody
      Expression of ZIP6 in rat hypothalamus
      Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-ZIP6/SLC39A6 (extracellular) Antibody (#AZT-006), (1:200), followed by goat anti-rabbit-AlexaFluor-488. ZIP6 immunoreactivity (green) appears in the median eminence (ME, arrows). Cell nuclei are stained with DAPI (blue). 3V = Third ventricle.
    Indirect flow cytometry
    • Anti-ZIP6/SLC39A6 (extracellular) Antibody
      Cell surface detection of ZIP6 in live intact human Jurkat T-cell leukemia cells:
      ___ Cells.
      ___ Cells + goat-anti-rabbit-FITC.
      ___ Cells + Anti-ZIP6/SLC39A6 (extracellular) Antibody (#AZT-006), (5 µg) + goat-anti-rabbit-FITC.
    References
    1. Matsui, C. et al. (2017) FEBS Lett. 591, 3348.
    2. Takatani-Nakase, T. (2018) Biol. Pharm. Bull. 41, 1517.
    3. Bin, B.H. et al. (2018) J. Immunol. Res. 2018, 9365747.
    4. Bagheri, S. et al. (2019) Monoclon. Antib. Immunodiagn. Immunother. 38, 70.
    5. Colomar-Carando, N. et al. (2019) J. Immunol. 202, 441.
    6. Taylor, K.M. et al. (2016) Biochem. J. 473, 2531.
    7. Kong, B.Y. et al. (2014) Mol. Hum. Reprod. 20, 1077.
    Scientific background

    ZIP6 (SLC39A6) is a zinc (Zn) transporter located on the cell membrane.

    Zn is an essential heavy metal, part of numerous biological processes as an enzymatic co-factor, and also as an intracellular signaling mediator that recruits extracellular stimuli and determines the final outputs of various physiological pathways. Intracellular and extracellular Zn levels regulate many cellular processes such as autophagy, cell growth and death, and cell migration1,2.

    Movement of Zn in and out of cells, across membranes, is regulated by zinc transporters. Zn transporter protein 6 (ZIP6) is a member of the ZIP family of Zinc transporters and of the LIV-1 sub-family. ZIP transporters contain 8 transmembrane domains with a cytoplasmic region between TM3 and TM4. LIV-1 subfamily members contain the HEXXH motif within TM5. They also exhibit diverse lengths of the N-terminal extracellular domain3.

    The gene encoding this transporter has been shown to take part in estrogen receptor‐positive breast cancer and serves as a reliable marker of this disease. Overexpression of ZIP6 protein is also observed in prostate and kidney tumor cells4.

    ZIP6 is also strongly upregulated during T-cell activation5. ZIP6 heteromerizes with ZIP10 for oocyte-to-egg transition and epithelial to mesenchymal transition in fetus development6,7.

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Last update: 24/02/2020

    Anti-ZIP6/SLC39A6 (extracellular) Antibody (#AZT-006) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot and indirect live cell flow cytometry applications. It has been designed to recognize LIV-1 from rat, mouse, and human samples.

    For research purposes only, not for human use
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