Overview
Type: Synthetic peptide
Form: Lyophilized powder
Aquaporin 1 Blocking Peptide (#BLP-QP001) is the original antigen used for immunization during Anti-Aquaporin 1 Antibody (#AQP-001) generation. The blocking peptide binds and ‘blocks’ Anti-Aquaporin 1 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat kidney membranes:1. Anti-Aquaporin 1 Antibody (AQP-001), (1:400).
2. Anti-Aquaporin 1 Antibody, preincubated with Aquaporin 1 Blocking Peptide (#BLP-QP001).
Expression of AQP1 in rat kidneyImmunohistochemical staining of paraffin-embedded rat kidney sections using Anti-Aquaporin 1 Antibody (#AQP-001), (1:100). AQP1 staining (brown) is present in both distal (DT) and proximal (PT) convoluted tubules in the renal cortex. Note that a weak staining is also present in collecting ducts (CD) while glomeruli (Gl) are negative. Hematoxilin is used as the counterstain.- Mouse paraffin-embedded kidney sections (1:100) (Kim, J.I. et al. (2013) Am. J. Physiol. 304, F1283.).
Properties
Sequence
- (C)KVWTSGQVEEYDLDADDIN corresponding to amino acid residues 242-260 of human AQP1 (Accession P29972).
Accession (Uniprot) Number P29972
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 μg peptide per 1 μg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
