Aquaporin 4/AQP4 (300-314) Blocking Peptide (#BLP-QP014) is the original antigen used for immunization during Anti-Aquaporin 4 (AQP4) (300-314) Antibody (#AQP-014) generation. The blocking peptide binds and ‘blocks’ Anti-Aquaporin 4/AQP4 (300-314) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
- Western blot analysis of mouse (lanes 1 and 3) and rat (lanes 2 and 4) brain lysates:
- Expression of Aquaporin 4 in rat brainImmunohistochemical staining of rat brain using Anti-Aquaporin 4 (AQP4) (300-314) Antibody (#AQP-014), (1:200). A. Aquaporin 4 (green) is detected in blood vessels in layers 1-3 of rat neocortex. B. Astrocytic processes were stained using mouse anti glial fibrillary acidic protein (GFAP) (red) and DAPI (blue) was used to delineate the cortical layers. C. Merge of Aquaporin 4 and GFAP shows overlap along blood vessels (arrows) in agreement with the role of astrocytes and Aquaporin 4 in regulation of the blood-brain barrier.
- (C)HVIDIDRGDEKKGKD, corresponding to amino acid residues 300-314 of rat AQP4 (Accession P47863).