Certificate of Analysis

ASIC3 Channel Premium Research Pack

All You Need for ASIC3 Channel Research
Cat #: ESP-201 13 Vials

Antibody Compounds

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat

Anti-ASIC3 Antibody Cat #: ASC-018

Size: 0.2 ml Source: Rabbit Type: Polyclonal Application: IC, IH, WB Reactivity: H, M, R
Immunogen
Peptide KPRSGLEEAQRRQAS(C), corresponding to amino acid residues 2-16 of rat ASIC3 (Accession O35240). Intracellular, N-terminus.
Homology
Mouse - 14/15 amino acid residues identical; human - 11/15 amino acid residues identical.

Guinea pig Anti-ASIC3 Antibody Cat #: AGP-052

Size: 0.2 ml Source: Guinea pig Type: Polyclonal Application: IH, WB Reactivity: M, R
Immunogen
Peptide KPRSGLEEAQRRQAS(C), corresponding to amino acid residues 2-16 of rat ASIC3 (Accession O35240). Intracellular, N-terminus.
Homology
Mouse - 14/15 amino acid residues identical; human - 11/15 amino acid residues identical.

Anti-pan ASIC (extracellular) Antibody Cat #: ASC-031

Size: 0.2 ml Source: Rabbit Type: Polyclonal Application: IC, IH, LCI, WB Reactivity: H, M, R
Immunogen
Peptide (C)TRYGKELSMVKIPSK, corresponding to amino acid residues 368-382 of rat ASIC1 (Accession P55926), amino acid residues 367–381 of rat ASIC2 (Accession Q62962), amino acid residues 375–388 of rat ASIC3 (Accession O35240) and amino acid residues 378–390 of rat ASIC4 (Accession Q9JHS6). Extracellular loop.
Homology
ASIC1: Mouse, rat, human – identical.
ASIC2: Mouse, rat, human – 14/15 amino acid residues identical.
ASIC3: Mouse, rat, human – 12/15 amino acid residues identical.
ASIC4: Mouse, rat – 12/15 amino acid residues identical; human – 11/15 amino acid residues identical.

ASIC3 Channel Overexpressed Membrane Fractions Cat #: LX-100

Size: 0.1 ml
Origin
Rat ASIC3 cDNA (NM_173135) was transcribed in vitro to produce full length mRNA. ASIC3 mRNA was injected into Xenopus oocytes and protein expression was verified by detection of pH evoked APETx2 (#RTA-100) sensitive ionic current. Membrane fraction was prepared from oocyte population homogenate and lyophilized. Non-injected oocytes underwent the same procedure excluding mRNA injection.

Non-Antibody Compounds

APETx2 Cat #: STA-160

Size: 0.1 mg Source: Synthetic MW: 4561 Da. Purity: >98% (HPLC) Net peptide content: 100%
Origin
Anthopleura elegantissima (Sea anemone).
Effective concentration
10 nM - 3 µM.
Activity
APETx2 inhibits ASIC3 channels1.
Storage before reconstitution
Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Reconstitution
Any aqueous buffer. Centrifuge all product preparations before use (10000 x g 5 min).
Storage after reconstitution
Up to one week at 4°C or three months at -20°C.

Cono AIVGRPRF-amide Cat #: STC-174

Size: 0.5 mg Source: Synthetic peptide MW: 914 Da. Purity: >98% (HPLC) Net peptide content: 100%
Origin
Conus textile.
Effective concentration
50 µM.
Activity
Cono AIVGRPRF-amide is an ASIC3 channel positive modulator.
Storage before reconstitution
Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Reconstitution
Any aqueous buffer. Centrifuge all product preparations before use (10000 x g for 5 min).
Storage after reconstitution
Up to two weeks at 4°C or three months at -20°C.

Cono RPRF-amide Cat #: STC-170

Size: 0.5 mg Source: Synthetic peptide MW: 574 Da. Purity: >98% (HPLC) Net peptide content: 100%
Origin
Conus textile.
Effective concentration
50 µM.
Activity
Cono RPRF-amide is an ASIC3 channel positive modulator1.
Storage before reconstitution
Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Reconstitution
Any aqueous buffer. Centrifuge all product preparations before use (10000 x g for 5 min).
Storage after reconstitution
Up to two weeks at 4°C or three months at -20°C.

Cono VGRPRF-amide Cat #: STC-172

Size: 0.5 mg Source: Synthetic peptide MW: 730 Da. Purity: >98% (HPLC) Net peptide content: 100%
Origin
Conus textile.
Effective concentration
50 µM.
Activity
Cono VGRPRF-amide is an ASIC3 channel positive modulator1.
Storage before reconstitution
Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Reconstitution
Any aqueous buffer. Centrifuge all product preparations before use (10000 x g for 5 min).
Storage after reconstitution
Up to two weeks at 4°C or three months at -20°C.

GMQ hydrochloride Cat #: G-165

Size: 50 mg Source: Synthetic MW: 237.1 Purity: >99%
Effective concentration
1-5 mM.
Activity
GMQ is an ASIC modulator, an ASIC3 opener and also induces an acidic shift of the pH dependence of inactivation of ASIC1a, -1b, -2a, and -31.
Storage before reconstitution
Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Reconstitution
50 mM in DMSO. Centrifuge all product preparations before use (10000 x g 5 min).
Storage after reconstitution
Up to four weeks at 4°C or three months at -20°C.

Antibody Storage & Reconstitution Instructions

Storage before reconstitution

Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.

Reconstitution

Add deionized water, depending on the sample size.

Storage after reconstitution

The reconstituted solution can be stored at 4°C for up to 2 weeks. For longer periods, small aliquots should be stored at -20°C or below.
Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).

Control antigen storage before reconstitution

Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.

Control antigen reconstitution

100 μl water.

Control antigen storage after reconstitution

-20ºC.

Preadsorption control

1 μg peptide per 1 μg antibody.

For more information, please refer to individual certificate of analysis for each product.

Note

A guinea pig polyclonal antibody (#AGP-052) is included in this Research Pack. Please take into account when reacting with a secondary antibody.

ASIC3 Channel Overexpressed Membrane Fractions includes:
1 x 0.1 ml lyophilized ASIC3 channel overexpressed membrane fractions
1 x 0.1 ml lyophilized non-injected Xenopus oocyte membrane fractions