ASIC5 (extracellular) Blocking Peptide (#BLP-SC032) is the original antigen used for immunization during Anti-ASIC5 (extracellular) Antibody (#ASC-032) generation. The blocking peptide binds and ‘blocks’ Anti-ASIC5 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
- Western blot analysis of rat brain (lanes 1 and 4), mouse brain (lanes 2 and 5) and rat small intestine (lanes 3 and 6) lysates:
- Expression of ASIC5 in mouse cortexImmunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-ASIC5 (extracellular) Antibody (#ASC-032), (1:200), followed by goat-anti-rabbit-AlexaFluor-488. ASIC5 immunoreactivity (green) is detected in cells of the pyramidal layer (arrows). Cell nuclei are stained with DAPI (blue).
- (C)RQLKTVHQEYPWGE, corresponding to amino acid residues 284-297 of mouse ASIC5 (Accession Q9R0Y1).