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BDNF Positive Control for Western Blot

Cat #: PCP-B250
Lyophilized Powder yes
Product Components: Recombinant human BDNF in Laemmli Buffer
Form Lyophilized powder. Reconstituted BDNF contains Laemmli Bufferx1
Amount 100 ng of BDNF. Sufficient for two SDS-PAGE lanes.
Content Recombinant human BDNF in Laemmli Buffer.
Storage before reconstitution The product ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 20 µl double distilled water (DDW).
BDNF concentration after reconstitution 5 ng/µl.
Standard quality control of each lot Western blot analysis.
Applications: wb
Positive control for Western blot
Western blot
  • Method of Use

    Dissolve the lyophilized powder by adding 20µl double distilled water (DDW). Heat the solubilized product to 1000C for 5 minutes. Load 10µl of the solution into an SDS-PAGE lane alongside the experimental samples. Run the gel and perform Western blot with an Anti-BDNF antibody as per your usual protocol. For example, our own Anti-BDNF Antibody (ANT-010).

  • Expected Result
    Single band detected at 14kD.
  • Western blot analysis using BDNF Positive Control
    Western blot analysis using BDNF Positive Control
    Lanes 1 and 2: 10µl (corresponding to 50ng) per lane of BDNF Positive Control for Western Blot (#PCP-B250), was run in a SDS-PAGE and detected in a Western blot using Anti-BDNF Antibody (#ANT-010), (1:200).

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot
Last update: 24/08/2022

BDNF Positive Control for Western Blot (#PCP-B250), is a recombinant, highly purified BDNF protein in a ready-to-use single reagent format. This makes it a good positive control by loading it alongside the experimental lysates or samples in a SDS-PAGE. This product enables to check whether the primary and secondary antibodies are working as expected and also visualize a reference of the expected molecular weight of the target protein.

For research purposes only, not for human use

Applications

Specifications

Scientific Background

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