Cat #: BLP-NT010
Size: 40 µg
Type: Synthetic peptide
Form: Lyophilized powder
For research purposes only, not for human use
- Western blot analysis with Anti-BDNF Antibody:1,5. Recombinant human BDNF protein (#B-250).
2,6. Recombinant mouse proBDNF protein (#B-240).
3,7. Recombinant human beta-NGF protein (#N-245).
4,8. Recombinant human Neurotrophin-3 (NT-3) protein (#N-260).
Lanes 1-4: Anti-BDNF Antibody (#ANT-010), (1:200).
Lanes 5-8: Anti-BDNF Antibody, preincubated with BDNF Blocking Peptide (#BLP-NT010).
Note that the antibody recognizes both BDNF and proBDNF but fails to recognize the closely related NGF and NT-3 neurotrophins.
- Mouse brain sections.
- Western blot analysis of 100 ng of each Recombinant human BDNF protein (#B-250) (lanes 1 and 5), Recombinant mouse proBDNF protein (#B-240) (lanes 2 and 6), Recombinant human Neurotrophin-3 (NT-3) protein (#N-260) (lanes 3 and 7) and Recombinant human beta-NGF protein (#N-245) (lanes 4 and 8):
- Mouse and rat brain sections (1:300-1:400).
- (C)VLEKVPVSKQLK, corresponding to amino acid residues 166-178 of human BDNF (precursor) (Accession P23560).
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Standard Quality Control Of Each Lot Western blot analysis.
Last update: 23/08/2020
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