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A Ligand of the Bombesin G-Protein Coupled Receptor

Cat #: GPB-100
Lyophilized Powder yes
  • Bioassay Tested
  • Origin Synthetic peptide originated from Bombina bombina (Fire-bellied toad).
    MW: 1638.88 Da
    Purity: >98% (HPLC)
    Effective concentration 1-100 nM.
    Modifications pyro-Glu1.
    Molecular formula C71H110N24O18S.
    CAS No.: 31362-50-2
    Activity Bombesin is a GPCR external ligand that mediates the inhibition of food intake and regulates the stress and fear response1.
    1. Casanueva, F.F. et al. (1996) Proc. Natl. Acad. Sci. U. S. A93, 1406.
    Shipping and storage Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
    Solubility Any aqueous buffer. Centrifuge all product preparations before use (10000 x g 5 min).
    Storage of solutions Up to one week at 4°C or three months at -20°C.
    Our bioassay
    • Alomone Labs Bombesin induces Ca2+ influx in 3T3-L1 cells.
      Alomone Labs Bombesin induces Ca2+ influx in 3T3-L1 cells.
      Cells were loaded with Fluo-3AM probe. Ca2+ influx was measured as a function of increasing Bombesin (#GPB-100) concentrations. The Ca2+ level is plotted against Bombesin concentrations (ED50 = 2.1 ng/ml).
    References - Scientific background
    1. Anastasi, A. (1971) Naunyn. Schmiedebergs. Arch. Pharmakol269, 135.
    2. Battey, J. and Wada, E. (1991) Trends Neurosci14, 524.
    3. Ohki-Hamazaki, H. et al. (2005) Int. J. Dev. Biol. 49, 293.
    4. Wada E. et al. (1991) Neuron 6, 421.
    5. Moody, T. W. and Merali, Z. (2004) Peptides 25, 511.
    Scientific background

    Bombesin is an active neuropeptide originally isolated from amphibian skin but also found in the central and peripheral nervous systems of amphibians1. Later it was established that mammals and birds express two family groups of endogenous Bombesin-like factors, the gastrin-releasing peptides and neuromedin B2. The biological activities of bombesin are mediated through a family of three specific G-protein coupled receptors (GPCRs): BB1 which is also known as a neuromedin B receptor, BB2 also known as a gastrin-releasing peptide receptor and BB3 which is a low affinity binder for the Bombesin-like factors3,4.

    Bombesin and Bombesin-like factors show a broad range of biological activities such as regulation of smooth muscle contraction, stimulation of neuropeptide and hormone secretion, modulation of neural activity, growth regulation, and mediate anorexic effects in the hypothalamus5.

    Target Bombesin receptors
    Peptide Content: 100%
    Last update: 02/01/2022

    Bombesin (#GPB-100) is a highly pure, synthetic, and biologically active protein.

    For research purposes only, not for human use
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