Overview
Type: Synthetic peptide
Form: Lyophilized Powder
C5aR2 (extracellular) Blocking Peptide (#BLP-AR034) is the original antigen used for immunization during Anti-C5aR2 (extracellular) Antibody (#AAR-034) generation. The blocking peptide binds and ‘blocks’ Anti-C5aR2 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat brain lysate (lanes 1 and 3) and mouse brain lysate (lanes 2 and 4):1-2. Anti-C5aR2 (extracellular) Antibody (#AAR-034), (1:400).
3-4. Anti-C5aR2 (extracellular) Antibody, preincubated with C5aR2 (extracellular) Blocking Peptide (BLP-AR034).
Western blot analysis of mouse J774 macrophage cell line lysate (lanes 1 and 3) and mouse M1 myeloid leukemia cell line lysate (lanes 2 and 4):1-2. Anti-C5aR2 (extracellular) Antibody (#AAR-034), (1:400).
3-4. Anti-C5aR2 (extracellular) Antibody, preincubated with C5aR2 (extracellular) Blocking Peptide (BLP-AR034).
Expression of C5aR2 in rat hippocampus.Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-C5aR2 (extracellular) Antibody (#AAR-034), (1: 300), followed by goat anti-rabbit-AlexaFluor-488. A. Staining in the hippocampal CA1 region, showed C5aR2 immunoreactivity (green) in glial profiles (arrows). B. Pre-incubation of the antibody with C5aR2 (extracellular) Blocking Peptide (BLP-AR034), suppressed staining. Cell nuclei are stained with DAPI (blue). P = pyramidal layer, SO = stratum oriens, SR = stratum radiatum.
Properties
Sequence
- Peptide (C)RRLLQEHYPPRLV(S)G, corresponding to amino acid residues 179 - 193 of mouse C5aR2 (Accession Q8BW93).
Accession (Uniprot) Number Q8BW93
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 2 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
