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Certificate of Analysis

Ca2+-Activated Cl Channel Antibody Explorer Kit

A Screening Package of Ca2+-Activated Cl Channel Antibodies Economically Priced

Cat #: AK-405
Size: 12 Vials
Form: Lyophilized

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat

Anti-TMEM16A (ANO1) (extracellular) Antibody Cat #: ACL-011

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IF, IH, WB Reactivity: M, R
Immunogen
Peptide (C)EYVKRKQRYEVDFNLE, corresponding to amino acid residues 679-694 of mouse Anoctamin-1 (Accession Q8BHY3). 3rd extracellular loop.
Homology
Rat - 15/16 amino acid residues identical; human - 14/16 amino acid residues identical.

TMEM16A/ANO1 (extracellular) Blocking Peptide Cat #: BLP-CL011

Size: 40 µg

Anti-TMEM16B (ANO2) (extracellular) Antibody Cat #: ACL-012

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IC, IF, IH, WB Reactivity: M, R
Immunogen
Peptide (C)HSKRPEQWDLDHSLE, corresponding to amino acid residues 632-646 of mouse Anoctamin-2 (Accession Q8CFW1). 3rd extracellular loop.
Homology
Rat - 14/15 amino acid residues identical; human - 13/15 amino acid residues identical.

TMEM16B/ANO2 (extracellular) Blocking Peptide Cat #: BLP-CL012

Size: 40 µg

Anti-Anoctamin-5 Antibody Cat #: ACL-015

Size: 50 µl Host: Rabbit Type: Polyclonal Application: WB Reactivity: M, R
Immunogen
Peptide (C)TKMEIPRTHQEYE, corresponding to amino acid residues 532-544 of mouse ANO5 (Accession Q75UR0). 2nd intracellular loop.
Homology
Rat – identical; human – 12/13 amino acid residues identical.

Anoctamin-5 Blocking Peptide Cat #: BLP-CL015

Size: 40 µg

Anti-Anoctamin-6 (extracellular) Antibody Cat #: ACL-016

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IFC, LCI, WB Reactivity: H, M, R
Immunogen
Peptide (C)KLPKNINGTDPIQK, corresponding to amino acid residues 487-500 of human ANO6 (Accession Q4KMQ2). 2nd extracellular loop.
Homology
Mouse, rat – 12/14 amino acid residues identical.

Anoctamin-6 (extracellular) Blocking Peptide Cat #: BLP-CL016

Size: 40 µg

Anti-Bestrophin-1 (extracellular) Antibody Cat #: ABC-001

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IFC, IH, LCI, WB Reactivity: H, M, R
Immunogen
Peptide (C)NPNKDYPGHEMD, corresponding to amino acid residues 259-270 of mouse Bestrophin-1 (Accession O88870). 3rd extracellular loop.
Homology
Rat - 10/12 amino acid residues identical; human - 9/12 amino acid residues identical.

Bestrophin-1 (extracellular) Blocking Peptide Cat #: BLP-BC001

Size: 40 µg

Anti-Bestrophin-2 (extracellular) Antibody Cat #: ABC-002

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IH, WB Reactivity: H, M, R
Immunogen
Peptide (C)DPAQGYKDHTLD, corresponding to amino acid residues 259-270 of mouse Bestrophin-2 (Accession Q8BGM5). 3rd extracellular loop.
Homology
Rat - identical; human - 11/12 amino acid residues identical.

Bestrophin-2 (extracellular) Blocking Peptide Cat #: BLP-BC002

Size: 40 µg

Antibody Storage & Reconstitution Instructions

Storage before reconstitution

Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.

Reconstitution

Add deionized water, depending on the sample size.

Storage after reconstitution

The reconstituted solution can be stored at 4°C for up to 2 weeks. For longer periods, small aliquots should be stored at -20°C or below.
Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).

Control antigen storage before reconstitution

Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.

Control antigen reconstitution

100 μl water.

Control antigen storage after reconstitution

-20ºC.

Preadsorption control

1 μg peptide per 1 μg antibody.

For research purposes only, not for human use
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