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Certificate of Analysis

Ca2+ ATPase Antibody Explorer Kit

A Screening Package of Ca2+ ATPase Antibodies Economically Priced

Cat #: AK-300
Size: 7 Vials
Form: Lyophilized

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat

Anti-PMCA1 Antibody Cat #: ACP-005

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IF, IH, WB Reactivity: H, M, R
Immunogen
Peptide (C)SGVKNSLKEANHD, corresponding to amino acid residues 12-24 of human PMCA1 (Accession P20020). Intracellular, N-terminus.
Homology
Rat, mouse - identical.

Anti-PMCA2 Antibody Cat #: ACP-002

Size: 50 µl Host: Rabbit Type: Polyclonal Application: WB Reactivity: M, R
Immunogen
Peptide (C)KEIPDPSSINAKTLE, corresponding to amino acid residues 522-536 of rat PMCA2 (Accession P11506). 2nd intracellular loop.
Homology
Mouse - identical; human - 13/15 amino acid residues identical.

Anti-PMCA3 Antibody Cat #: ACP-003

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IF, IH, WB Reactivity: M, R
Immunogen
Peptide (C)EFHPKPQ(N)QREVPH, corresponding to amino acid residues 10-23 of rat PMCA3 (Accession Q64568). Intracellular, N-terminus.
Homology
Rat, mouse – 13/14 amino acid residues identical; human – 11/14 amino acid residues identical.

Anti-PMCA4 Antibody Cat #: ACP-004

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IF, IH, WB Reactivity: R
Immunogen
Peptide CDRILNKEGGIVPFKTK, corresponding to amino acid residues 601-617 of rat PMCA4 (Accession Q64542). 2nd intracellular loop.
Homology
Mouse – 12/17 amino acid residues identical.

Anti-SERCA1 Antibody Cat #: ACP-011

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IC, IF, IH, WB Reactivity: M, R
Immunogen
Peptide (C)TPDQVKRHLEKYG, corresponding to amino acid residues 25-37 of rat SERCA1 (Accession Q64578). Cytoplasmic, N-terminus.
Homology
Mouse - identical; human - 12/13 amino acid residues identical.

Anti-SERCA2 Antibody Cat #: ACP-012

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IC, IF, WB Reactivity: H, M, R
Immunogen
Peptide CTPNKPSRTSMSK, corresponding to amino acid residues 498-510 of rat SERCA2 (Accession P11507). 2nd cytoplasmic loop.
Homology
Mouse, human - identical.

Anti-SERCA3 Antibody Cat #: ACP-014

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IF, IH, WB Reactivity: M, R
Immunogen
Peptide (C)DEALKYLSRHHVD, corresponding to amino acid residues 981-993 of rat SERCA3 (Accession P18596). Cytoplasmic, C-terminus.
Homology
Mouse – 11/13 amino acid residues identical; human – 10/13 amino acid residues identical.

Antibody Storage & Reconstitution Instructions

Storage before reconstitution

Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.

Reconstitution

Add deionized water, depending on the sample size.

Storage after reconstitution

The reconstituted solution can be stored at 4°C for up to 2 weeks. For longer periods, small aliquots should be stored at -20°C or below.
Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).

Control antigen storage before reconstitution

Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.

Control antigen reconstitution

100 μl water.

Control antigen storage after reconstitution

-20ºC.

Preadsorption control

1 μg peptide per 1 μg antibody.

For research purposes only, not for human use
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