Overview
Type: Synthetic peptide
Form: Lyophilized powder
CACNA2D2/Cavα2δ2 (extracellular) Blocking Peptide (#BLP-CC102) is the original antigen used for immunization during Anti-CACNA2D2 (CaVα2δ2) (extracellular) Antibody (#ACC-102) generation. The blocking peptide binds and ‘blocks’ Anti-CACNA2D2/Cavα2δ2 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat lung (lanes 1 and 6), rat brain (lanes 2 and 7), mouse brain (lanes 3 and 8), rat heart (lanes 4 and 9) and mouse heart (lanes 5 and 10) lysates:1-5. Anti-CACNA2D2 (CaVα2δ2) (extracellular) Antibody (#ACC-102), (1:200).
6-10. Anti-CACNA2D2 (CaVα2δ2) (extracellular) Antibody, preincubated with CACNA2D2/Cavα2δ2 (extracellular) Blocking Peptide (#BLP-CC102).
Expression of CaVα2δ2 channel in rat cerebellumImmunohistochemical staining rat cerebellum using Anti-CACNA2D2 (CaVα2δ2) (extracellular) Antibody (#ACC-102). A. CaVα2δ2 (green) appears in the soma of Purkinje cells (arrow) and in the molecular layer (M). B. Preincubation of the antibody with the blocking peptide blocks staining of Purkinje cells and molecular layer. DAPI is used as the counterstain (blue).
Properties
Sequence
- (C)DLEAWAEKFKVLASNR, corresponding to amino acid residues 850-865 of rat CaVα2δ2 (Accession Q8CFG6).
Accession (Uniprot) Number Q8CFG6
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
