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Certificate of Analysis

CACNA2D3/Cavα2δ3 (extracellular) Blocking Peptide

Overview

Cat #: BLP-CC103
Size: 40 µg
Type: Synthetic peptide
Form: Lyophilized powder
Applications: bl

Application key:

BL- Blocking
For research purposes only, not for human use

Properties

Properties

Sequence
  • CSWWHSDMTAKAQK, corresponding to amino acid residues 942-955 of rat CaVα2δ3 (Accession Q8CFG5).
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.

Applications

Applications

Blocking
  • Western blot analysis of rat brain (lanes 1 and 3) and K562 (lanes 2 and 4) lysates:
    Western blot analysis of rat brain (lanes 1 and 3) and K562 (lanes 2 and 4) lysates:
    1,2. Anti-CACNA2D3 (CaVα2δ3) (extracellular) Antibody (#ACC-103), (1:200).
    3,4. Anti-CACNA2D3 (CaVα2δ3) (extracellular) Antibody, preincubated with CACNA2D3/Cavα2δ3 (extracellular) Blocking Peptide (#BLP-CC103).
  • Rat brain.
  • Rat hippocampus and neocortex.
  • Expression of CaVα2δ3 in rat hippocampus and cortex
    Expression of CaVα2δ3 in rat hippocampus and cortex
    Immunohistochemical staining of rat hippocampal CA3 region (A) and rat neocortex (B) using Anti-CACNA2D3 (CaVα2δ3) (extracellular)-ATTO Fluor-594 Antibody (#ACC-103-AR). In both, A and B, CaVα2δ3 staining (red) appears in pyramidal neurons (arrows). DAPI is used as the counterstain (blue).
Last update: 11/04/2021
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