Overview
Type: Synthetic peptide
Form: Lyophilized powder
CACNB2 Blocking Peptide (#BLP-CC105) is the original antigen used for immunization during Anti-CACNB2 Antibody (#ACC-105) generation. The blocking peptide binds and ‘blocks’ Anti-CACNB2 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain membranes:1,2. Anti-CACNB2 Antibody (#ACC-105), (1:800).
3,4. Anti-CACNB2 Antibody, preincubated with CACNB2 Blocking Peptide (#BLP-CC105).
Expression of CaVβ2 in mouse hippocampus.Immunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-CACNB2 Antibody (#ACC-105), (1:200), followed by goat anti-rabbit-AlexaFluor-488. A. Staining in the mouse CA3 hippocampal region, showed immunoreactivity (green) in neurons (horizontal arrows) of the CA3 pyramidal layer (P). B. Pre-incubation of the antibody with CACNB2 Blocking Peptide (#BLP-CC105), suppressed staining. Cell nuclei are stained with DAPI (blue).
Expression of CaVβ2 in mouse heart.Immunohistochemical staining of frozen mouse heart sections with Anti-CACNB2 Antibody (#ACC-105), followed by goat anti-rabbit-AlexaFluor-488. A. CaVβ2 immunoreactivity (green) appears in striate muscle fibers (horizontal arrows). B. Pre-incubation of the antibody with CACNB2 Blocking Peptide (#BLP-CC105), suppressed staining. Cell nuclei are stained with DAPI (blue).
Properties
Sequence
- (C)HEHVDRYVPHREHNHRE, corresponding to amino acid residues 571-587 of rat CACNB2 (Accession Q8VGC3).
Accession (Uniprot) Number Q8VGC3
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
