Overview
Type: Synthetic peptide
Form: Lyophilized powder
CACNG3 Blocking Peptide (#BLP-CC113) is the original antigen used for immunization during Anti-CACNG3 Antibody (#ACC-113) generation. The blocking peptide binds and ‘blocks’ Anti-CACNG3 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat brain (lanes 1 and 4), mouse brain (lanes 2 and 5) and human neuroblastoma (SH-SY5Y) cells (lanes 3 and 6):1-3. Anti-CACNG3 Antibody (#ACC-113), (1:1000).
4-6. Anti-CACNG3 Antibody, preincubated with CACNG3 Blocking Peptide (#BLP-CC113).
Properties
Sequence
- (C)RSHSELLKKSTFAR, corresponding to amino acid residues 210-223 of rat CACNG3 (Accession Q8VHX0).
Accession (Uniprot) Number Q8VHX0
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
