Calnexin Blocking Peptide (#BLP-CS009) is the original antigen used for immunization during Anti-Calnexin Antibody (#ACS-009) generation. The blocking peptide binds and ‘blocks’ Anti-Calnexin primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
- Western blot analysis of rat heart membrane (lanes 1 and 5), mouse heart membrane (lanes 2 and 6), human breast adenocarcinoma (MCF7) lysate (lanes 3 and 7) and human ovary adenocarcinoma (OVCAR-3) cell line lysate (lanes 4 and 8):
- Expression of Calnexin in mouse hippocampusImmunohistochemical staining of mouse hippocampal CA1 region using Anti-Calnexin Antibody (#ACS-009), (1:200). A. Canx staining (green) appears in neurons (horizontal arrow) and astrocytes (vertical arrow). B. GFAP staining of astrocytic fibers (red). C. Merge of panels A and B demonstrates co-localization in some astrocytes. DAPI is used to stain nuclei (blue).
- (C)KAEEDEILNRSPRNRK, corresponding to amino acid residues 572-587 of rat Canx (Accession P35565).