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Camptothecin

Topoisomerase Inhibitor
Cat #: C-330
Lyophilized Powder yes
  • Bioassay Tested
  • Origin Camptotheca acuminata.
    Source Natural
    MW: 348.36
    Purity: >99%
    Effective concentration 1-300 nM.
    Structure
    Chemical name (S)-4-Ethyl-4-hydroxy-1H-pyrano-[3',4':6,7]indolizino[1,2-b]quinoline-3,14(4H,12H)-dione.
    Molecular formula C20H16N2O4.
    CAS No.: 7689-03-4.
    Activity Camptothecin is an alkaloid that specifically inhibits DNA topoisomerase I/II and is an anticancer agent1,2. It also promotes apoptosis in a caspase-dependent manner3.
    References-Activity
    1. Hsiang, Y.H. et al. (1985) J. Biol. Chem260, 14873.
    2. Capranico, G. et al. (1990) Nucleic Acids Res18, 6611.
    3. Cotter, T.G. et al. (1992) Anticancer Res12, 773.
    Shipping and storage Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
    Solubility DMSO. Centrifuge all product preparations before use (10000 x g 5 min).
    Storage of solutions Up to two weeks at 4°C or six months at -20°C.
    Our bioassay
    • Alomone Labs Camptothecin inhibits the proliferation of MC-7 breast cancer cells.
      Alomone Labs Camptothecin inhibits the proliferation of MC-7 breast cancer cells.
      Cells were treated with different concentrations of Camptothecin (#C-330) for 4 days. The number of live cells was measured by XTT cell proliferation assay kit, normalized to the control (100%) and plotted against the drug concentration.
    References - Scientific background
    1. Hsiang, Y.H. et al.  (1985) J. Biol. Chem260, 14873.
    2. Capranico, G. et al. (1990) Nucleic Acids Res18, 6611.
    3. Chen, A.Y., and Liu, L.F. (1994) Annu. Rev. Pharmacol. Toxicol. 34, 191.
    4. Tanizawa, A. et al. (1993) J. Biol. Chem268, 25463.
    5. Wang, J.C. (1996) Annu. Rev. Biochem65, 635.
    6. Kaufmann, S.H. (1998) Biochim. Biophys. Acta. 1400, 195.
    7. Pommier, Y. et al. (1998) Biochim. Biophys. Acta. 1400, 83.
    8. Jaxel, C. et al. (1991) J. Biol. Chem266, 20418.
    9. Li, C.J. et al. (1994) J. Biol. Chem. 269, 7051.
    10. Shao, R.G. et al. (1997) J. Biol. Chem272, 31321.
    Scientific background

    Camptothecin is an anti-cancer alkaloid that specifically inhibits DNA topoisomerase I and DNA topoisomerase II (topoisomerase I/II).1-3 The highly conserved region around Gly505 of topoisomerase I was found to be important for camptothecin activity.4

    Camptothecin converts topoisomerase into a cell poison by trapping the cleavable covalent complex of enzyme with duplex DNA and blocking the re-ligation step. Thereby enhancing and stabilizing the formation of DNA breakage which is responsible for cell death.1-9

    Camptothecin inhibits (IC50 = 30 nM) Tat protein-mediated transactivation of HIV-1 and enhances PKCa activity during apoptosis in HL60 myeloid leukemia cells via a caspase-regulated mechanism.9,10

    Last update: 16/08/2020

    Camptothecin (#C-330) is a highly pure, natural, and biologically active compound.

    For research purposes only, not for human use

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    Scientific Background

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