Cav1.1/CACNA1S (extracellular) Blocking Peptide (#BLP-CC314) is the original antigen used for immunization during Anti-CaV1.1 (CACNA1S) (extracellular) Antibody (#ACC-314) generation. The blocking peptide binds and ‘blocks’ Anti-Cav1.1/CACNA1S (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
- Western blot analysis of rat skeletal muscle:
- Expression of CaV1.1 in rat skeletal muscleImmunohistochemical staining of paraffin-embedded rat skeletal muscle sections using Anti-CaV1.1 (CACNA1S) (extracellular) Antibody (#ACC-314), (1:100), followed by DAB. CACNA1S staining is highly specific for striated muscle cells. Note that smooth muscle cells of arteries are not stained (arrow). Hematoxilin is used as the counterstain.
- CGNVDPDESARIS, corresponding to amino acid residues 506-518 of rat CaV1.1 (Accession Q02485).