Overview
Type: Synthetic peptide
Form: Lyophilized powder
Cav1.1/CACNA1S (extracellular) Blocking Peptide (#BLP-CC314) is the original antigen used for immunization during Anti-CaV1.1 (CACNA1S) (extracellular) Antibody (#ACC-314) generation. The blocking peptide binds and ‘blocks’ Anti-Cav1.1/CACNA1S (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat skeletal muscle:1. Anti-CaV1.1 (CACNA1S) (extracellular) Antibody (#ACC-314), (1:200).
2. Anti-CaV1.1 (CACNA1S) (extracellular) Antibody, preincubated with Cav1.1/CACNA1S (extracellular) Blocking Peptide (#BLP-CC314).
Expression of CaV1.1 in rat skeletal muscleImmunohistochemical staining of paraffin-embedded rat skeletal muscle sections using Anti-CaV1.1 (CACNA1S) (extracellular) Antibody (#ACC-314), (1:100), followed by DAB. CACNA1S staining is highly specific for striated muscle cells. Note that smooth muscle cells of arteries are not stained (arrow). Hematoxilin is used as the counterstain.
Properties
Sequence
- CGNVDPDESARIS, corresponding to amino acid residues 506-518 of rat CaV1.1 (Accession Q02485).
Accession (Uniprot) Number Q02485
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
