Certificate of Analysis

CaV1.2 (CACNA1C) Channel Antibody and Membrane Fractions Kit

Kit Contains Anti-CaV1.2 (CACNA1C) Antibody and all Controls Necessary for Robust Western Blot Analysis
Cat #: LK-104A 3 Vials

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat

Anti-CaV1.2 (CACNA1C) Antibody Cat #: ACC-003

Size: 0.2 ml Host: Rabbit Type: Polyclonal Application: IC, IF, IFC, IH, IP, WB Reactivity: H, M, R
Immunogen
Peptide (C)TTKINMDDLQPSENEDKS, corresponding to amino acid residues 848-865 of rat CaV1.2 (Accession P22002). Intracellular loop between domains II and III.
Homology
Rat, mouse - 8/11 amino acid residues identical.

CaV1.2 (CACNA1C) Channel Overexpressing Membrane Fraction Cat #: LX-104

Size: 0.2 ml
Origin
Rabbit CaV1.2 cDNA (M57974.1) was transcribed in vitro to produce full length mRNA. CaV1.2 mRNA was injected into Xenopus oocytes and protein expression was verified by detecting voltage dependent Nifedipine (#N-120) sensitive ionic current. Membrane fraction was prepared from oocyte population homogenate and lyophilized. Non-injected oocytes underwent the same procedure excluding mRNA injection.

Antibody Storage & Reconstitution Instructions

Storage before reconstitution

Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.

Reconstitution

Add deionized water, depending on the sample size.

Storage after reconstitution

The reconstituted solution can be stored at 4°C for up to 2 weeks. For longer periods, small aliquots should be stored at -20°C or below.
Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).

Control antigen storage before reconstitution

Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.

Control antigen reconstitution

100 μl water.

Control antigen storage after reconstitution

-20ºC.

Preadsorption control

1 μg peptide per 1 μg antibody.

Note
Kit includes:
1 x 40 µg Anti-CaV1.2 (CACNA1C) Antibody control antigen
1 x 0.1 ml lyophilized non-injected Xenopus oocyte membrane lysate