Overview
Type: Synthetic peptide
Form: Lyophilized powder
Cav2.3/CACNA1E Blocking Peptide (#BLP-CC006) is the original antigen used for immunization during Anti-CaV2.3 (CACNA1E) Antibody (#ACC-006) generation. The blocking peptide binds and ‘blocks’ Anti-Cav2.3/CACNA1E primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat brain membranes:1. Anti-CaV2.3 (CACNA1E) Antibody (#ACC-006), (1:200).
2. Anti-CaV2.3 (CACNA1E) Antibody, preincubated with Cav2.3/CACNA1E Blocking Peptide (#BLP-CC006).
Expression of CaV2.3 in mouse cerebellumImmunohistochemical staining of mouse cerebellum with Anti-CaV2.3 (CACNA1E) Antibody (#ACC-006). CaV2.3 (red) appears in both soma and dendritic trees of several Purkinje cells. Staining of neurofilament 200 (green) in the same brain section demonstrates the relationship between axons passing through the granule layer and the Purkinje cells.
Properties
Sequence
- (C)SASQERSLDEGVSIDG, corresponding to amino acid residues 892-907 of rat CaV2.3 (Accession Q07652).
Accession (Uniprot) Number Q07652
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
