CNGA4 Blocking Peptide (#BLP-PC074) is the original antigen used for immunization during Anti-CNGA4 Antibody (#APC-074) generation. The blocking peptide binds and ‘blocks’ Anti-CNGA4 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
- Western blot analysis of rat brain lysate:
- Expression of CNGA4 in mouse olfactory bulbImmunohistochemical staining of CNGA4 channel in mouse olfactory bulb using Anti-CNGA4 Antibody (#APC-074). A. CNGA4 staining (green) appears in glomeruli (horizontal arrows) and outside the glomeruli (vertical arrows). To define the location of glomeruli (G), the same section was stained with mouse anti-synaptophysin (red) and the nissl stain DAPI (blue). B. Pre-incubation of Anti-CNGA4 Antibody with the peptide antigen blocks the staining in the olfactory bulb.
- (C) SQDGKVKTTESTPPAPTK, corresponding to amino acid residues 2-19 of rat CNGA4 (Accession Q64359).