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Certificate of Analysis

Connexin Antibody Explorer Kit

A Screening Package of Connexin Antibodies for Economically Priced

Cat #: AK-420
Size: 9 Vials
Form: Lyophilized

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat

Anti-Connexin-26 (GJB2) Antibody Cat #: ACC-212

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IC, IF, IH, WB Reactivity: H, M, R
Immunogen
Peptide (C)HEKKRKFMKGEIK, corresponding to amino acid residues 100-112 of rat Connexin-26 (Accession P21994). Intracellular, cytoplasmic loop.
Homology
Mouse - identical; human - 12/13 amino acid residues identical.

Anti-Connexin-29 (extracellular) Antibody Cat #: ACC-223

Size: 50 µl Host: Rabbit Type: Polyclonal Application: WB Reactivity: M, R
Immunogen
Peptide (C)GNDENEFI(S)HLGQPG, corresponding to amino acid residues 56-70 of mouse Connexin-29 (Accession Q921C1). 1st extracellular loop.
Homology
Mouse – 14/15 amino acid residues identical; rat – 13/15 amino acid residues identical.

Anti-Connexin-31 (GJB3) Antibody Cat #: ACC-213

Size: 50 µl Host: Rabbit Type: Polyclonal Application: WB Reactivity: M, R
Immunogen
Peptide (C)EINKLLSEQDGSLK, corresponding to amino acid residues 216-228 of mouse Connexin-31 (Accession P28231). Intracellular, C-terminus
Homology
Rat – 11/13 amino acid residues identical.

Anti-Connexin-32 Antibody Cat #: ACC-211

Size: 50 µl Host: Rabbit Type: Polyclonal Application: WB Reactivity: H, M, R
Immunogen
Peptide (C)EINKLLSEQDGSLK, corresponding to amino acid residues 247-260 of rat Connexin-32 (Accession P08033). Intracellular, C-terminus.
Homology
Human, mouse – identical.

Anti-Connexin-36 Antibody Cat #: ACC-209

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IF, IH, WB Reactivity: M, R
Immunogen
Peptide (C)EVKELAPHPSGLR, corresponding to amino acid residues 170-182 of rat Connexin-36 (Accession O70610). Intracellular loop.
Homology
Mouse, human - 12/13 amino acid residues identical.

Anti-Connexin-37 Antibody Cat #: ACC-204

Size: 50 µl Host: Rabbit Type: Polyclonal Application: WB Reactivity: H, M, R
Immunogen
Peptide (C)EHQMAKISVAEDGR, corresponding to amino acid residues 131-144 of rat Connexin-37 (Accession Q03190). Intracellular loop.
Homology
Mouse – identical; human – 13/14 amino acid residues identical.

Anti-Connexin-40 (GJA5) Antibody Cat #: ACC-205

Size: 50 µl Host: Rabbit Type: Polyclonal Application: WB Reactivity: M, R
Immunogen
Peptide (C)GHRFPQGYHSDKR, corresponding to amino acid residues 328-340 of rat Connexin-40 (Accession P28234). Intracellular, C-terminus.
Homology
Mouse - 12/13 amino acid residues identical; human - 11/13 amino acid residues identical.

Anti-Connexin-43 Antibody Cat #: ACC-201

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IC, IF, IH, WB Reactivity: H, M, R
Immunogen
Peptide (C)HAQPFDFPDDNQNSK, corresponding to amino acids residues 331-345 of human Connexin-43 (Accession P17302). Intracellular, C-terminus.
Homology
Rat - 14/15 amino acid residues identical; mouse - 13/15 amino acid residues identical.

Anti-Connexin-45 (GJC1) Antibody Cat #: ACC-207

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IF, IH, WB Reactivity: H, M, R
Immunogen
Peptide (C)KEQSQPKPKHDGRR, corresponding to amino acid residues 153-166 of rat Connexin-45 (Accession A4GG66). Intracellular loop.
Homology
Mouse, human - identical.

Antibody Storage & Reconstitution Instructions

Storage before reconstitution

Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.

Reconstitution

Add deionized water, depending on the sample size.

Storage after reconstitution

The reconstituted solution can be stored at 4°C for up to 2 weeks. For longer periods, small aliquots should be stored at -20°C or below.
Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).

Control antigen storage before reconstitution

Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.

Control antigen reconstitution

100 μl water.

Control antigen storage after reconstitution

-20ºC.

Preadsorption control

1 μg peptide per 1 μg antibody.

For research purposes only, not for human use
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