This product is freeze dried. All water molecules have been removed.
Every lot is tried & tested in a relevant biological assay.
It was shown to inhibit synaptic activation of mGluR1 in Purkinje cells cerebellar slices with IC50 of 37µM2.
Alomone Labs CPCCOEt inhibits mGluR1-mediated Ca2+ mobilization in U2OS cells.Dose-response of CPCCOEt (#C-370) normalized inhibition of human mGluR1-mediated, L-Glutamate-evoked Ca2+ mobilization. hmGluR1-expressing cells were loaded with calcium-sensitive dye, incubated with a varying CPCCOEt concentrations, and stimulated by 5 µM L-Glutamate (EC80). Changes in intracellular Ca2+ following stimulation were detected as changes in maximum relative fluorescence (RLU) using FLIPRTETRA™.
CPCCOEt, is a compound that acts as a selective, non-competitive antagonist of mGluR1. It can inhibit mGluR1 signaling without affecting glutamate binding1. CPCCOEt selectively inhibits mGluR1b-induced increases in intracellular calcium and demonstrates IC50 values of 6.5 µM1.
Glutamate is the most abundant excitatory neurotransmitter in the central nervous system and it modulates activity of many types of synapses by activating in part metabotropic glutamate receptors (mGluRs), members of G-protein coupled receptors (GPCRs). These receptors are divided into three groups based on sequence homology with a total of eight subtypes, mGluR1 to mGluR8.
mGluR1 from group I, plays an important role in the central sensitization of pain and in a variety of physiological functions including regulation of ion channels, synaptic transmission, and synaptic plasticity1,2.
CPCCOEt (#C-370) is a highly pure, synthetic, and biologically active compound.