CRF1/CRHR1 (extracellular) Blocking Peptide (#BLP-CR050) is the original antigen used for immunization during Anti-CRF1/CRHR1 (extracellular) Antibody (#ACR-050) generation. The blocking peptide binds and ‘blocks’ Anti-CRF1/CRHR1 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
- Western blot analysis of mouse brain membrane (lanes 1 and 4), rat brain membrane (lane 2 and 5) and rat cortex lysate (lane 3 and 6):
- Western blot analysis of human neuroblastoma (SH-SY5Y) (lanes 1 and 4), human brain astrocytoma (CCF-STTGI) (lanes 2 and 5) and rat pheochromocytoma (PC12) (lanes 3 and 6):
- Expression of Corticotropin-releasing factor receptor 1 in mouse brainImmunohistochemical staining of mouse hippocampus and mouse cerebellum using Anti-CRF1/CRHR1 (extracellular) Antibody (#ACR-050), (1:600). A. CRF1 staining (green) in the cerebellum appears in cerebellar molecular layer (MOL). B. CRF1 staining (green) in the hippocampus appears in soma (horizontal arrows) and dendrites of pyramidal neurons (arrow). In both panels DAPI was used as the counterstain.
- ENLSLTSNVSGLQC, corresponding to amino acid residues 31-44 of rat CRHR1 (Accession P35353).