Overview
Type: Synthetic peptide
Form: Lyophilized powder
CRMP1 Blocking Peptide (#BLP-IP028) is the original antigen used for immunization during Anti-CRMP1 Antibody (#AIP-028) generation. The blocking peptide binds and ‘blocks’ Anti-CRMP1 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only, not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of mouse brain (lanes 1 and 5), rat cortex (lanes 2 and 6), rat new born brain (lanes 3 and 7) and human brain glioblastoma (U-87 MG) cell line lysates:1-4. Anti-CRMP1 Antibody (#AIP-028), (1:200).
5-8. Anti-CRMP1 Antibody, preincubated with CRMP1 Blocking Peptide (#BLP-IP028).
Properties
Sequence
- (C)RVRIRSKVFGLHSVSR, corresponding to amino acid residues 481-496 of rat CRMP1 (Accession Q62950).
Accession (Uniprot) Number Q62950
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
